Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16700 | 3' | -54.5 | NC_004156.1 | + | 197099 | 0.67 | 0.964836 |
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Target: 5'- -cGGCUGCGCAGACUU-GAUucaGCGACa -3' miRNA: 3'- guCUGGUGCGUCUGAGuCUGc--UGCUG- -5' |
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| 16700 | 3' | -54.5 | NC_004156.1 | + | 199627 | 0.71 | 0.824109 |
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Target: 5'- uCGGcAUCACGCAGAC--GGACGAgGACc -3' miRNA: 3'- -GUC-UGGUGCGUCUGagUCUGCUgCUG- -5' |
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| 16700 | 3' | -54.5 | NC_004156.1 | + | 206722 | 0.69 | 0.916161 |
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Target: 5'- -cGACUAUGCAGAgUUuGcCGACGACu -3' miRNA: 3'- guCUGGUGCGUCUgAGuCuGCUGCUG- -5' |
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| 16700 | 3' | -54.5 | NC_004156.1 | + | 207250 | 0.7 | 0.891637 |
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Target: 5'- gGGAau-CGUAGACUCGGACGAUuACg -3' miRNA: 3'- gUCUgguGCGUCUGAGUCUGCUGcUG- -5' |
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| 16700 | 3' | -54.5 | NC_004156.1 | + | 211179 | 1.11 | 0.005013 |
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Target: 5'- aCAGACCACGCAGACUCAGACGACGACg -3' miRNA: 3'- -GUCUGGUGCGUCUGAGUCUGCUGCUG- -5' |
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| 16700 | 3' | -54.5 | NC_004156.1 | + | 214184 | 0.67 | 0.964836 |
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Target: 5'- -uGACCGUGCAGAUUCAGAguugcCGucuCGGCu -3' miRNA: 3'- guCUGGUGCGUCUGAGUCU-----GCu--GCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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