Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16716 | 3' | -41.9 | NC_004166.1 | + | 1361 | 0.66 | 0.99996 |
Target: 5'- uGGGGAaaGAUGGuuGGAuGAGCuggauGCa -3' miRNA: 3'- cCUCCU--UUACUuuUCUuCUCGuuu--CG- -5' |
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16716 | 3' | -41.9 | NC_004166.1 | + | 38879 | 0.66 | 0.999959 |
Target: 5'- uGAGGAAcuUGAAGAcacaaucGAAGAGCuGGAGg -3' miRNA: 3'- cCUCCUUu-ACUUUU-------CUUCUCG-UUUCg -5' |
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16716 | 3' | -41.9 | NC_004166.1 | + | 3763 | 0.66 | 0.999915 |
Target: 5'- aGGGcGGGGAUGAugauuuAGAGGAGgaugauccaaaugcCGGAGCc -3' miRNA: 3'- -CCU-CCUUUACUuu----UCUUCUC--------------GUUUCG- -5' |
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16716 | 3' | -41.9 | NC_004166.1 | + | 39359 | 0.66 | 0.999894 |
Target: 5'- -aAGGAAagGGAAuacGGAGGGGUAaaacguGAGCa -3' miRNA: 3'- ccUCCUUuaCUUU---UCUUCUCGU------UUCG- -5' |
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16716 | 3' | -41.9 | NC_004166.1 | + | 36626 | 0.66 | 0.999894 |
Target: 5'- aGAGGAGAacauGAGGAAGAuCAGAGa -3' miRNA: 3'- cCUCCUUUacu-UUUCUUCUcGUUUCg -5' |
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16716 | 3' | -41.9 | NC_004166.1 | + | 26582 | 0.66 | 0.999891 |
Target: 5'- aGGGGGuacacauucauAGGAGGGCAAGGa -3' miRNA: 3'- -CCUCCuuuacuuu---UCUUCUCGUUUCg -5' |
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16716 | 3' | -41.9 | NC_004166.1 | + | 14929 | 0.67 | 0.999809 |
Target: 5'- cGGAGuaacGAGcAUGugGGGAAGAGUuacGAGCa -3' miRNA: 3'- -CCUC----CUU-UACuuUUCUUCUCGu--UUCG- -5' |
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16716 | 3' | -41.9 | NC_004166.1 | + | 33748 | 0.67 | 0.999746 |
Target: 5'- gGGAGGAcggcauAGGAAG-GCAAGGg -3' miRNA: 3'- -CCUCCUuuacuuUUCUUCuCGUUUCg -5' |
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16716 | 3' | -41.9 | NC_004166.1 | + | 12239 | 0.67 | 0.999739 |
Target: 5'- aGGAGGAugccacAUGGAA---GGAGCAcgcuuacAGGCg -3' miRNA: 3'- -CCUCCUu-----UACUUUucuUCUCGU-------UUCG- -5' |
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16716 | 3' | -41.9 | NC_004166.1 | + | 35345 | 0.68 | 0.999439 |
Target: 5'- aGGAGGAAA---AAGGAAauGAGCGAAu- -3' miRNA: 3'- -CCUCCUUUacuUUUCUU--CUCGUUUcg -5' |
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16716 | 3' | -41.9 | NC_004166.1 | + | 8891 | 0.68 | 0.999424 |
Target: 5'- aGAGGAAGaaaagaaagccucUGAGAagcgucAGAAGGGCGcacagAAGCc -3' miRNA: 3'- cCUCCUUU-------------ACUUU------UCUUCUCGU-----UUCG- -5' |
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16716 | 3' | -41.9 | NC_004166.1 | + | 36839 | 0.68 | 0.99885 |
Target: 5'- -cAGGAuuuaGAcAGGaAAGGGCAGAGCg -3' miRNA: 3'- ccUCCUuua-CUuUUC-UUCUCGUUUCG- -5' |
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16716 | 3' | -41.9 | NC_004166.1 | + | 2890 | 0.69 | 0.99853 |
Target: 5'- -aAGGGcaucaugGGUGAAGAGAcacAGAaaGCAGAGCu -3' miRNA: 3'- ccUCCU-------UUACUUUUCU---UCU--CGUUUCG- -5' |
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16716 | 3' | -41.9 | NC_004166.1 | + | 33486 | 0.69 | 0.998214 |
Target: 5'- aGGGGuGAGcgGGAuGGAAGaAGUuuGGCg -3' miRNA: 3'- -CCUC-CUUuaCUUuUCUUC-UCGuuUCG- -5' |
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16716 | 3' | -41.9 | NC_004166.1 | + | 3713 | 0.7 | 0.996044 |
Target: 5'- aGAGGAAAUGAAccaauacgcuGAAauGCAGGGCa -3' miRNA: 3'- cCUCCUUUACUUuu--------CUUcuCGUUUCG- -5' |
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16716 | 3' | -41.9 | NC_004166.1 | + | 11759 | 0.7 | 0.99525 |
Target: 5'- aGAGGAcGUgGAAGAGAAGGagcuaguguuGCGGGGUa -3' miRNA: 3'- cCUCCUuUA-CUUUUCUUCU----------CGUUUCG- -5' |
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16716 | 3' | -41.9 | NC_004166.1 | + | 1869 | 0.7 | 0.99525 |
Target: 5'- aGAGGAGAUugu--GAAGcguGCAAAGCg -3' miRNA: 3'- cCUCCUUUAcuuuuCUUCu--CGUUUCG- -5' |
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16716 | 3' | -41.9 | NC_004166.1 | + | 38350 | 0.7 | 0.994332 |
Target: 5'- -aAGGAuggcGUGAAAAGAAGAGUuaauAAGg -3' miRNA: 3'- ccUCCUu---UACUUUUCUUCUCGu---UUCg -5' |
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16716 | 3' | -41.9 | NC_004166.1 | + | 11827 | 0.71 | 0.990694 |
Target: 5'- gGGAGGAAAUGAAcccGGAG-GCcAAGa -3' miRNA: 3'- -CCUCCUUUACUUuu-CUUCuCGuUUCg -5' |
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16716 | 3' | -41.9 | NC_004166.1 | + | 6195 | 0.71 | 0.990694 |
Target: 5'- cGGAGGAAuUGAAAuGGcgucagguuauGGAGUAAGuGCa -3' miRNA: 3'- -CCUCCUUuACUUUuCU-----------UCUCGUUU-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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