Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16716 | 3' | -41.9 | NC_004166.1 | + | 5328 | 0.72 | 0.988114 |
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Target: 5'- uGAGGaAAAUGAGAAGcgcGAGCAAgacgaaccuacaaucAGCa -3' miRNA: 3'- cCUCC-UUUACUUUUCuu-CUCGUU---------------UCG- -5' |
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| 16716 | 3' | -41.9 | NC_004166.1 | + | 38162 | 0.73 | 0.975206 |
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Target: 5'- uGGAGGAAAgagccgccaGAGAAGcGGAG-AAAGCu -3' miRNA: 3'- -CCUCCUUUa--------CUUUUCuUCUCgUUUCG- -5' |
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| 16716 | 3' | -41.9 | NC_004166.1 | + | 22233 | 0.74 | 0.956088 |
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Target: 5'- aGGAGGucacUGAGuuuugGAGAuAGGGCAGAGUg -3' miRNA: 3'- -CCUCCuuu-ACUU-----UUCU-UCUCGUUUCG- -5' |
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| 16716 | 3' | -41.9 | NC_004166.1 | + | 36165 | 0.83 | 0.581961 |
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Target: 5'- --uGGAAAUGGAAAGAAGGGCGAugacuauuuaaGGCc -3' miRNA: 3'- ccuCCUUUACUUUUCUUCUCGUU-----------UCG- -5' |
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| 16716 | 3' | -41.9 | NC_004166.1 | + | 24379 | 1.17 | 0.007372 |
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Target: 5'- uGGAGGAAAUGAAAAGAAGAGCAAAGCc -3' miRNA: 3'- -CCUCCUUUACUUUUCUUCUCGUUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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