Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16716 | 3' | -41.9 | NC_004166.1 | + | 39359 | 0.66 | 0.999894 |
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Target: 5'- -aAGGAAagGGAAuacGGAGGGGUAaaacguGAGCa -3' miRNA: 3'- ccUCCUUuaCUUU---UCUUCUCGU------UUCG- -5' |
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| 16716 | 3' | -41.9 | NC_004166.1 | + | 36626 | 0.66 | 0.999894 |
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Target: 5'- aGAGGAGAacauGAGGAAGAuCAGAGa -3' miRNA: 3'- cCUCCUUUacu-UUUCUUCUcGUUUCg -5' |
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| 16716 | 3' | -41.9 | NC_004166.1 | + | 3763 | 0.66 | 0.999915 |
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Target: 5'- aGGGcGGGGAUGAugauuuAGAGGAGgaugauccaaaugcCGGAGCc -3' miRNA: 3'- -CCU-CCUUUACUuu----UCUUCUC--------------GUUUCG- -5' |
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| 16716 | 3' | -41.9 | NC_004166.1 | + | 38879 | 0.66 | 0.999959 |
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Target: 5'- uGAGGAAcuUGAAGAcacaaucGAAGAGCuGGAGg -3' miRNA: 3'- cCUCCUUu-ACUUUU-------CUUCUCG-UUUCg -5' |
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| 16716 | 3' | -41.9 | NC_004166.1 | + | 1361 | 0.66 | 0.99996 |
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Target: 5'- uGGGGAaaGAUGGuuGGAuGAGCuggauGCa -3' miRNA: 3'- cCUCCU--UUACUuuUCUuCUCGuuu--CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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