Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16970 | 5' | -51.1 | NC_004323.1 | + | 44657 | 0.66 | 0.973426 |
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Target: 5'- -aAAGugGGCCAGCACacuacguacgacguGgUCGGCAAGc -3' miRNA: 3'- ucUUCugCUGGUUGUG--------------CgAGUCGUUU- -5' |
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| 16970 | 5' | -51.1 | NC_004323.1 | + | 57358 | 0.68 | 0.934383 |
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Target: 5'- ----uGCGACCGACACGaaCAGUAAAa -3' miRNA: 3'- ucuucUGCUGGUUGUGCgaGUCGUUU- -5' |
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| 16970 | 5' | -51.1 | NC_004323.1 | + | 38131 | 0.69 | 0.923284 |
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Target: 5'- cGGcGGACGugCAgaugaGCGCGgaCAGCGAAa -3' miRNA: 3'- -UCuUCUGCugGU-----UGUGCgaGUCGUUU- -5' |
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| 16970 | 5' | -51.1 | NC_004323.1 | + | 28570 | 0.7 | 0.897867 |
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Target: 5'- gGGAAGuC-AUCAACACGCUCAaGCAc- -3' miRNA: 3'- -UCUUCuGcUGGUUGUGCGAGU-CGUuu -5' |
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| 16970 | 5' | -51.1 | NC_004323.1 | + | 46005 | 0.7 | 0.883584 |
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Target: 5'- uGAAGACGACgagcgcguCAACAUGUUUAGUAGu -3' miRNA: 3'- uCUUCUGCUG--------GUUGUGCGAGUCGUUu -5' |
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| 16970 | 5' | -51.1 | NC_004323.1 | + | 13261 | 0.71 | 0.852052 |
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Target: 5'- ---uGACcACCGACACGUUUAGCGGAc -3' miRNA: 3'- ucuuCUGcUGGUUGUGCGAGUCGUUU- -5' |
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| 16970 | 5' | -51.1 | NC_004323.1 | + | 88888 | 0.72 | 0.767868 |
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Target: 5'- cGGAAGACGacuuguuGCCGACuCGCagCAGCAAAc -3' miRNA: 3'- -UCUUCUGC-------UGGUUGuGCGa-GUCGUUU- -5' |
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| 16970 | 5' | -51.1 | NC_004323.1 | + | 121146 | 0.76 | 0.577118 |
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Target: 5'- uGGAAGACGGCCAcCACGCac-GCAAAa -3' miRNA: 3'- -UCUUCUGCUGGUuGUGCGaguCGUUU- -5' |
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| 16970 | 5' | -51.1 | NC_004323.1 | + | 57476 | 1.07 | 0.008294 |
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Target: 5'- aAGAAGACGACCAACACGCUCAGCAAAa -3' miRNA: 3'- -UCUUCUGCUGGUUGUGCGAGUCGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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