Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16971 | 5' | -51.6 | NC_004323.1 | + | 60304 | 0.66 | 0.992583 |
Target: 5'- aUGGUguaGUCGGGcGuGUCGUUGUUu -3' miRNA: 3'- gGCCAag-CAGCCCuCuUAGCAGCAAc -5' |
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16971 | 5' | -51.6 | NC_004323.1 | + | 63283 | 0.67 | 0.977692 |
Target: 5'- -aGGUUCGcUGGcGAu-AUCGUCGUUGa -3' miRNA: 3'- ggCCAAGCaGCC-CUcuUAGCAGCAAC- -5' |
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16971 | 5' | -51.6 | NC_004323.1 | + | 113988 | 0.68 | 0.966507 |
Target: 5'- uCCGaGUUUGUCGacaaGGAAUCGUCGgUGg -3' miRNA: 3'- -GGC-CAAGCAGCcc--UCUUAGCAGCaAC- -5' |
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16971 | 5' | -51.6 | NC_004323.1 | + | 62079 | 1.1 | 0.007353 |
Target: 5'- uCCGGUUCGUCGGGAGAAUCGUCGUUGu -3' miRNA: 3'- -GGCCAAGCAGCCCUCUUAGCAGCAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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