Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16972 | 3' | -41.3 | NC_004323.1 | + | 15350 | 0.66 | 1 |
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Target: 5'- ------aUACUGGCuGUGCAAugUUGg -3' miRNA: 3'- gauauaaAUGAUCG-CGCGUUugAAUg -5' |
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| 16972 | 3' | -41.3 | NC_004323.1 | + | 28968 | 0.66 | 1 |
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Target: 5'- aUAUGUagGCgucguGCGCGguAACUUGa -3' miRNA: 3'- gAUAUAaaUGau---CGCGCguUUGAAUg -5' |
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| 16972 | 3' | -41.3 | NC_004323.1 | + | 111269 | 0.69 | 0.999999 |
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Target: 5'- -----aUUGgUAGCGCGCGcACUUGg -3' miRNA: 3'- gauauaAAUgAUCGCGCGUuUGAAUg -5' |
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| 16972 | 3' | -41.3 | NC_004323.1 | + | 17291 | 0.69 | 0.999998 |
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Target: 5'- -----aUUAUUAGCGCGCAcgucGCUaGCa -3' miRNA: 3'- gauauaAAUGAUCGCGCGUu---UGAaUG- -5' |
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| 16972 | 3' | -41.3 | NC_004323.1 | + | 80067 | 0.7 | 0.999995 |
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Target: 5'- ------aUAUUGGCGCGCAGcACggACg -3' miRNA: 3'- gauauaaAUGAUCGCGCGUU-UGaaUG- -5' |
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| 16972 | 3' | -41.3 | NC_004323.1 | + | 64093 | 0.7 | 0.99999 |
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Target: 5'- -------aGCUGGCGaCGCGAGCcgGCa -3' miRNA: 3'- gauauaaaUGAUCGC-GCGUUUGaaUG- -5' |
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| 16972 | 3' | -41.3 | NC_004323.1 | + | 93182 | 0.7 | 0.999986 |
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Target: 5'- -----aUUACaUAGCGUGCGGGCUguuUGCg -3' miRNA: 3'- gauauaAAUG-AUCGCGCGUUUGA---AUG- -5' |
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| 16972 | 3' | -41.3 | NC_004323.1 | + | 44452 | 0.74 | 0.999384 |
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Target: 5'- --uUGUUUACguuuuCGCGUAAACUUGCg -3' miRNA: 3'- gauAUAAAUGauc--GCGCGUUUGAAUG- -5' |
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| 16972 | 3' | -41.3 | NC_004323.1 | + | 64987 | 1.14 | 0.037799 |
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Target: 5'- gCUAUAUUUACUAGCGCGCAAACUUACa -3' miRNA: 3'- -GAUAUAAAUGAUCGCGCGUUUGAAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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