Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16975 | 3' | -56.2 | NC_004323.1 | + | 64921 | 0.66 | 0.921739 |
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Target: 5'- aCCGUCG-C-CACGCGCgACaacaGGAUgGAu -3' miRNA: 3'- -GGCAGCaGcGUGUGCG-UGg---CCUAgCU- -5' |
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| 16975 | 3' | -56.2 | NC_004323.1 | + | 10663 | 0.66 | 0.921739 |
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Target: 5'- aCCGUagccaGgCGCgauuuggauuACGgGCGCCGGAUCGu -3' miRNA: 3'- -GGCAg----CaGCG----------UGUgCGUGGCCUAGCu -5' |
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| 16975 | 3' | -56.2 | NC_004323.1 | + | 118410 | 0.66 | 0.891515 |
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Target: 5'- gCGUCGUguUGgACACGaCGCCGGuuUCGu -3' miRNA: 3'- gGCAGCA--GCgUGUGC-GUGGCCu-AGCu -5' |
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| 16975 | 3' | -56.2 | NC_004323.1 | + | 77618 | 0.67 | 0.884795 |
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Target: 5'- gCGUCGUUGUACguuaGCGCAgacaCGGAUgcCGAc -3' miRNA: 3'- gGCAGCAGCGUG----UGCGUg---GCCUA--GCU- -5' |
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| 16975 | 3' | -56.2 | NC_004323.1 | + | 99238 | 0.67 | 0.884795 |
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Target: 5'- gUCGUacauuaCGUCGCGCACGguCCGcGGcCGGc -3' miRNA: 3'- -GGCA------GCAGCGUGUGCguGGC-CUaGCU- -5' |
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| 16975 | 3' | -56.2 | NC_004323.1 | + | 34558 | 0.67 | 0.855783 |
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Target: 5'- gUCGUCGUCGuCACGCGCucGCCGccuaaUGAg -3' miRNA: 3'- -GGCAGCAGC-GUGUGCG--UGGCcua--GCU- -5' |
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| 16975 | 3' | -56.2 | NC_004323.1 | + | 29208 | 0.68 | 0.806512 |
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Target: 5'- gCUGUgugccCGUCGC--GCGUACCGGAgugCGAa -3' miRNA: 3'- -GGCA-----GCAGCGugUGCGUGGCCUa--GCU- -5' |
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| 16975 | 3' | -56.2 | NC_004323.1 | + | 110908 | 0.68 | 0.806512 |
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Target: 5'- uCCGUCGgCGagggaaaaAUACGCAUCGGG-CGAa -3' miRNA: 3'- -GGCAGCaGCg-------UGUGCGUGGCCUaGCU- -5' |
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| 16975 | 3' | -56.2 | NC_004323.1 | + | 96672 | 0.69 | 0.761169 |
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Target: 5'- -aGUUGUCGCACAUGCAaUUGGcgCGc -3' miRNA: 3'- ggCAGCAGCGUGUGCGU-GGCCuaGCu -5' |
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| 16975 | 3' | -56.2 | NC_004323.1 | + | 99163 | 1.11 | 0.00233 |
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Target: 5'- cCCGUCGUCGCACACGCACCGGAUCGAa -3' miRNA: 3'- -GGCAGCAGCGUGUGCGUGGCCUAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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