Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16978 | 3' | -46.4 | NC_004323.1 | + | 37744 | 0.66 | 0.999962 |
Target: 5'- uGCGGCGCAAuuaaaaaauuuccuCGUCGUUGUUaaUAAUCu -3' miRNA: 3'- -UGCUGCGUUu-------------GCAGCAGUAAg-AUUGG- -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 21303 | 0.66 | 0.999957 |
Target: 5'- aACGGgGCcgugcCGUCGuUCAUUUUAGCUc -3' miRNA: 3'- -UGCUgCGuuu--GCAGC-AGUAAGAUUGG- -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 120739 | 0.66 | 0.999957 |
Target: 5'- gACGGCGCG--UGUUGUCAccauuuauUUUUGACg -3' miRNA: 3'- -UGCUGCGUuuGCAGCAGU--------AAGAUUGg -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 13511 | 0.66 | 0.999924 |
Target: 5'- aGCGGCGC---UGUgGUUAUUUUAACg -3' miRNA: 3'- -UGCUGCGuuuGCAgCAGUAAGAUUGg -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 18522 | 0.67 | 0.999868 |
Target: 5'- uAUGGCGCAAACGU-GUUcgAUUagaaUAACCc -3' miRNA: 3'- -UGCUGCGUUUGCAgCAG--UAAg---AUUGG- -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 119318 | 0.67 | 0.999829 |
Target: 5'- gACGugGUAGAUGaCGUCAacuguuuuauacUUCaagAACCa -3' miRNA: 3'- -UGCugCGUUUGCaGCAGU------------AAGa--UUGG- -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 86617 | 0.67 | 0.99978 |
Target: 5'- gAUGAaacgGCAAACGUUGcggaGUUCUAACUc -3' miRNA: 3'- -UGCUg---CGUUUGCAGCag--UAAGAUUGG- -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 88182 | 0.67 | 0.999718 |
Target: 5'- gACGucCGCAGACGaUGUCGUUaaucguGCCg -3' miRNA: 3'- -UGCu-GCGUUUGCaGCAGUAAgau---UGG- -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 40472 | 0.67 | 0.999675 |
Target: 5'- uACGACGCuuGuucaccaccguguacGCGUCGUCGUc--GGCCg -3' miRNA: 3'- -UGCUGCGu-U---------------UGCAGCAGUAagaUUGG- -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 40248 | 0.68 | 0.999549 |
Target: 5'- uUGGCGCAuGCuGUCGUUggugAUUCgcGCCa -3' miRNA: 3'- uGCUGCGUuUG-CAGCAG----UAAGauUGG- -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 59677 | 0.68 | 0.999549 |
Target: 5'- cCGGCGUggGCGUCGUCuacacUUUUAu-- -3' miRNA: 3'- uGCUGCGuuUGCAGCAGu----AAGAUugg -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 48290 | 0.68 | 0.999435 |
Target: 5'- uUGACGCAAuggagGCGUUGUUuaaaUCUAcACCa -3' miRNA: 3'- uGCUGCGUU-----UGCAGCAGua--AGAU-UGG- -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 53976 | 0.68 | 0.999435 |
Target: 5'- cCGACGCccGCGaCGUUcgAUUCUGGCg -3' miRNA: 3'- uGCUGCGuuUGCaGCAG--UAAGAUUGg -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 113992 | 0.69 | 0.998415 |
Target: 5'- -aGACGuCAAGaGUCGUUAUUUgcGCCg -3' miRNA: 3'- ugCUGC-GUUUgCAGCAGUAAGauUGG- -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 33889 | 0.7 | 0.996145 |
Target: 5'- -aGACGCAAGCGcuUCGa-GUUUUGGCCc -3' miRNA: 3'- ugCUGCGUUUGC--AGCagUAAGAUUGG- -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 9162 | 0.7 | 0.996145 |
Target: 5'- aACGGCGauGACGUCGUCGcugUCguugucacugGACCa -3' miRNA: 3'- -UGCUGCguUUGCAGCAGUa--AGa---------UUGG- -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 68118 | 0.71 | 0.992819 |
Target: 5'- gGCGGCGCcacuacccAAugGUCGUCG-UCgccGCCu -3' miRNA: 3'- -UGCUGCG--------UUugCAGCAGUaAGau-UGG- -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 70990 | 0.73 | 0.974493 |
Target: 5'- uUGACGCGcACGUCGUCucuguugauUUCUGuauaGCCu -3' miRNA: 3'- uGCUGCGUuUGCAGCAGu--------AAGAU----UGG- -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 65597 | 0.74 | 0.961541 |
Target: 5'- gGCGGCGUuuccguCGUCGUCAUUCUc--- -3' miRNA: 3'- -UGCUGCGuuu---GCAGCAGUAAGAuugg -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 75627 | 0.74 | 0.957697 |
Target: 5'- uGCGGCGUAAAUG-CGUCAUauUCUGuagauCCg -3' miRNA: 3'- -UGCUGCGUUUGCaGCAGUA--AGAUu----GG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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