miRNA: 3'- -UGCUGCGU-----UUGCAGCaGUAaGAUUGG- -5'
miRNA: 3'- -UGCugCGUuuGCAGCAGuaAGA-UUGG- -5'
miRNA: 3'- ugCugCGUUUGCAGCAGUaAGAUUGG- -5'
miRNA: 3'- -UGCUGCGUUUGCAgcAGUAAGAUUGG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16978 | 3' | -46.4 | NC_004323.1 | + | 110455 | 0.75 | 0.939729 |
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Target: 5'- aACGAUGCAaacauGAUGUCGaCGUgCUAGCCu -3' miRNA: 3'- -UGCUGCGU-----UUGCAGCaGUAaGAUUGG- -5' |
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| 16978 | 3' | -46.4 | NC_004323.1 | + | 117141 | 0.75 | 0.93457 |
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Target: 5'- aACGugGCGu-CGUCGUCcgUUUcGACCa -3' miRNA: 3'- -UGCugCGUuuGCAGCAGuaAGA-UUGG- -5' |
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| 16978 | 3' | -46.4 | NC_004323.1 | + | 61560 | 0.84 | 0.564844 |
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Target: 5'- -gGugGUAAACGUCGUCGaUUUAACCa -3' miRNA: 3'- ugCugCGUUUGCAGCAGUaAGAUUGG- -5' |
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| 16978 | 3' | -46.4 | NC_004323.1 | + | 21104 | 0.95 | 0.15855 |
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Target: 5'- aACGACGCAAACGUgcUCAUUCUAACCg -3' miRNA: 3'- -UGCUGCGUUUGCAgcAGUAAGAUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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