Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16983 | 5' | -54.1 | NC_004323.1 | + | 126894 | 0.66 | 0.937376 |
Target: 5'- --cUGAGgcgaaucugGGUCGGCGGAcgGAUCGCu -3' miRNA: 3'- uuuACUCg--------CCGGCCGCUUaaCUGGCG- -5' |
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16983 | 5' | -54.1 | NC_004323.1 | + | 13082 | 0.66 | 0.926922 |
Target: 5'- uAAUGAGUuaaauaGGCgGGC-AGUUGuCCGCu -3' miRNA: 3'- uUUACUCG------CCGgCCGcUUAACuGGCG- -5' |
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16983 | 5' | -54.1 | NC_004323.1 | + | 1038 | 0.67 | 0.88951 |
Target: 5'- ---cGAGCGGgUGGuCGA--UGACCaGCg -3' miRNA: 3'- uuuaCUCGCCgGCC-GCUuaACUGG-CG- -5' |
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16983 | 5' | -54.1 | NC_004323.1 | + | 106205 | 0.67 | 0.887406 |
Target: 5'- --uUGAGCgacaucacuuccauGGCUgaaGGCGAAUUaGCCGCu -3' miRNA: 3'- uuuACUCG--------------CCGG---CCGCUUAAcUGGCG- -5' |
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16983 | 5' | -54.1 | NC_004323.1 | + | 120030 | 0.69 | 0.790216 |
Target: 5'- ---cGA-CGG-CGGCGAGUUGGCgGCa -3' miRNA: 3'- uuuaCUcGCCgGCCGCUUAACUGgCG- -5' |
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16983 | 5' | -54.1 | NC_004323.1 | + | 63866 | 0.97 | 0.020971 |
Target: 5'- cAAAUGA-CGGCCGGCGAAUUGACCGCg -3' miRNA: 3'- -UUUACUcGCCGGCCGCUUAACUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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