Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17000 | 5' | -60.3 | NC_004333.2 | + | 40386 | 0.7 | 0.262347 |
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Target: 5'- gCGGCCGacuCGAaCcCGGUUGaaGACGGGCa -3' miRNA: 3'- -GCCGGC---GCUaGaGCCGACa-CUGCCCG- -5' |
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| 17000 | 5' | -60.3 | NC_004333.2 | + | 41839 | 0.67 | 0.374855 |
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Target: 5'- cCGGCCGCGAg--CGGU-----CGGGCa -3' miRNA: 3'- -GCCGGCGCUagaGCCGacacuGCCCG- -5' |
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| 17000 | 5' | -60.3 | NC_004333.2 | + | 45192 | 0.68 | 0.341901 |
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Target: 5'- cCGGCgaGCG-UCUaCGGCauuccguUGACGGGCg -3' miRNA: 3'- -GCCGg-CGCuAGA-GCCGac-----ACUGCCCG- -5' |
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| 17000 | 5' | -60.3 | NC_004333.2 | + | 45404 | 0.7 | 0.230883 |
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Target: 5'- aGGCCGCagaccuggcacucGGUUUCGGCggcgGcGACGGcGCa -3' miRNA: 3'- gCCGGCG-------------CUAGAGCCGa---CaCUGCC-CG- -5' |
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| 17000 | 5' | -60.3 | NC_004333.2 | + | 45435 | 1.11 | 0.000206 |
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Target: 5'- gCGGCCGCGAUCUCGGCUGUGACGGGCa -3' miRNA: 3'- -GCCGGCGCUAGAGCCGACACUGCCCG- -5' |
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| 17000 | 5' | -60.3 | NC_004333.2 | + | 46144 | 0.7 | 0.249611 |
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Target: 5'- gGGCgucgaCGUGAUCgaugCGGCcGUGAcguCGGGCa -3' miRNA: 3'- gCCG-----GCGCUAGa---GCCGaCACU---GCCCG- -5' |
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| 17000 | 5' | -60.3 | NC_004333.2 | + | 47903 | 0.67 | 0.383423 |
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Target: 5'- -cGCCGUGAccgugCUCGGCggcGUGACGaccacaucgaacGGCa -3' miRNA: 3'- gcCGGCGCUa----GAGCCGa--CACUGC------------CCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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