Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17002 | 3' | -55.5 | NC_004333.2 | + | 40345 | 0.69 | 0.482785 |
Target: 5'- -gCAGGCCGcgucgaucUgGCGAGCGAGCUaaacuacucguccGCGCu -3' miRNA: 3'- gaGUCUGGC--------AgCGCUUGCUCGA-------------CGUG- -5' |
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17002 | 3' | -55.5 | NC_004333.2 | + | 10501 | 0.69 | 0.483816 |
Target: 5'- -cCGGGCCGgcCGaCGAGCGGGUcggGCGCg -3' miRNA: 3'- gaGUCUGGCa-GC-GCUUGCUCGa--CGUG- -5' |
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17002 | 3' | -55.5 | NC_004333.2 | + | 15755 | 0.68 | 0.536578 |
Target: 5'- --gAGACacUCGUcAGCGAGCUGCGCg -3' miRNA: 3'- gagUCUGgcAGCGcUUGCUCGACGUG- -5' |
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17002 | 3' | -55.5 | NC_004333.2 | + | 40299 | 0.66 | 0.668848 |
Target: 5'- -cCAGA-CGUggaCGCGAugGGGCgaGCGCg -3' miRNA: 3'- gaGUCUgGCA---GCGCUugCUCGa-CGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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