Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17005 | 3' | -52.4 | NC_004333.2 | + | 2891 | 0.66 | 0.835617 |
Target: 5'- gCGAGGCuGAuGuUGGCcgagacgugcUGCGCAGCGGu- -3' miRNA: 3'- -GCUUCG-CUuC-ACCG----------ACGCGUUGCUug -5' |
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17005 | 3' | -52.4 | NC_004333.2 | + | 22908 | 0.66 | 0.835617 |
Target: 5'- aGAAGCGcGGUGcugccgcucgaGUUGUGCAGCGuAUa -3' miRNA: 3'- gCUUCGCuUCAC-----------CGACGCGUUGCuUG- -5' |
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17005 | 3' | -52.4 | NC_004333.2 | + | 40315 | 0.66 | 0.820606 |
Target: 5'- uGggGCGAgcgcgagcuGGaaaUGGCcgGCGCAggccgcgucgaucugGCGAGCg -3' miRNA: 3'- gCuuCGCU---------UC---ACCGa-CGCGU---------------UGCUUG- -5' |
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17005 | 3' | -52.4 | NC_004333.2 | + | 41297 | 0.66 | 0.816767 |
Target: 5'- uCGccGCGAuccgcucgaUGGCUGCGCAAUGcaguGCg -3' miRNA: 3'- -GCuuCGCUuc-------ACCGACGCGUUGCu---UG- -5' |
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17005 | 3' | -52.4 | NC_004333.2 | + | 36603 | 0.66 | 0.816767 |
Target: 5'- uCGAcaaucAGCGcauGUGGCaaucgcgucUGCGCAacGCGGGCu -3' miRNA: 3'- -GCU-----UCGCuu-CACCG---------ACGCGU--UGCUUG- -5' |
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17005 | 3' | -52.4 | NC_004333.2 | + | 21022 | 0.66 | 0.816767 |
Target: 5'- aCGGGcGaCGAAGUGGCcGCGCAgugcugcuccuGgGGACu -3' miRNA: 3'- -GCUU-C-GCUUCACCGaCGCGU-----------UgCUUG- -5' |
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17005 | 3' | -52.4 | NC_004333.2 | + | 17862 | 0.66 | 0.807024 |
Target: 5'- gCGuauGGCG-GGUGGUgucgcagGCGCGACGGc- -3' miRNA: 3'- -GCu--UCGCuUCACCGa------CGCGUUGCUug -5' |
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17005 | 3' | -52.4 | NC_004333.2 | + | 38967 | 0.67 | 0.776661 |
Target: 5'- gGAuGCGAAGUcGG-UGCGCAccGCGAu- -3' miRNA: 3'- gCUuCGCUUCA-CCgACGCGU--UGCUug -5' |
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17005 | 3' | -52.4 | NC_004333.2 | + | 27552 | 0.67 | 0.766203 |
Target: 5'- cCGggGCGAAu---CUGCGCAuUGAACa -3' miRNA: 3'- -GCuuCGCUUcaccGACGCGUuGCUUG- -5' |
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17005 | 3' | -52.4 | NC_004333.2 | + | 45101 | 0.67 | 0.764093 |
Target: 5'- gCGAGGCGAuacucgacgccUGuCUGCGCGGCGAucGCg -3' miRNA: 3'- -GCUUCGCUuc---------ACcGACGCGUUGCU--UG- -5' |
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17005 | 3' | -52.4 | NC_004333.2 | + | 41588 | 0.67 | 0.755596 |
Target: 5'- gCGgcGCGccGUGGCgGCGauGCGGGCu -3' miRNA: 3'- -GCuuCGCuuCACCGaCGCguUGCUUG- -5' |
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17005 | 3' | -52.4 | NC_004333.2 | + | 29768 | 0.67 | 0.73399 |
Target: 5'- cCGAucGCGAGGc-GUUGCGCuuCGGACg -3' miRNA: 3'- -GCUu-CGCUUCacCGACGCGuuGCUUG- -5' |
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17005 | 3' | -52.4 | NC_004333.2 | + | 2427 | 0.68 | 0.711945 |
Target: 5'- gCGGcAGCGugccuUGGUacgGCGCGGCGAACa -3' miRNA: 3'- -GCU-UCGCuuc--ACCGa--CGCGUUGCUUG- -5' |
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17005 | 3' | -52.4 | NC_004333.2 | + | 38594 | 0.68 | 0.700789 |
Target: 5'- cCGGcuGGCGcugaAGGUGGCg--GCAACGAACu -3' miRNA: 3'- -GCU--UCGC----UUCACCGacgCGUUGCUUG- -5' |
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17005 | 3' | -52.4 | NC_004333.2 | + | 26287 | 0.68 | 0.689562 |
Target: 5'- aGAacGGCGcccaauuGG-GGCuaUGCGCAGCGAACc -3' miRNA: 3'- gCU--UCGCu------UCaCCG--ACGCGUUGCUUG- -5' |
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17005 | 3' | -52.4 | NC_004333.2 | + | 6301 | 0.69 | 0.666942 |
Target: 5'- aCGAGcGCGcuGGUgaaaGGCUGCGCAGCcgugaGGACg -3' miRNA: 3'- -GCUU-CGCu-UCA----CCGACGCGUUG-----CUUG- -5' |
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17005 | 3' | -52.4 | NC_004333.2 | + | 41305 | 0.69 | 0.655573 |
Target: 5'- aCGAGGCcGAGcucgcccGGC-GCGCGAUGGACg -3' miRNA: 3'- -GCUUCGcUUCa------CCGaCGCGUUGCUUG- -5' |
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17005 | 3' | -52.4 | NC_004333.2 | + | 29882 | 0.7 | 0.564707 |
Target: 5'- uCGucGGCGAAGc-GCUGCGCGAuCGGGCc -3' miRNA: 3'- -GCu-UCGCUUCacCGACGCGUU-GCUUG- -5' |
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17005 | 3' | -52.4 | NC_004333.2 | + | 39478 | 0.7 | 0.564707 |
Target: 5'- -aAGGCGAAGaUGGaagcgcgcgGCGUAACGGACg -3' miRNA: 3'- gcUUCGCUUC-ACCga-------CGCGUUGCUUG- -5' |
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17005 | 3' | -52.4 | NC_004333.2 | + | 8900 | 0.7 | 0.553513 |
Target: 5'- uGAucuuGGCGAGGuUGGCgucgaUGCGCGACGcGGCu -3' miRNA: 3'- gCU----UCGCUUC-ACCG-----ACGCGUUGC-UUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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