Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17005 | 3' | -52.4 | NC_004333.2 | + | 21440 | 0.72 | 0.492279 |
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Target: 5'- aCGggGCGccgacgcaaugggucAAGUuccuGGCcgGCGCGGCGGGCa -3' miRNA: 3'- -GCuuCGC---------------UUCA----CCGa-CGCGUUGCUUG- -5' |
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| 17005 | 3' | -52.4 | NC_004333.2 | + | 44479 | 0.72 | 0.466991 |
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Target: 5'- gGgcGCGAAGUGGaUGaaggaaauCGCAGCGAACc -3' miRNA: 3'- gCuuCGCUUCACCgAC--------GCGUUGCUUG- -5' |
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| 17005 | 3' | -52.4 | NC_004333.2 | + | 20978 | 0.73 | 0.40691 |
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Target: 5'- gCGgcGCGGgcgAGUGGgUGCGUuuCGAGCg -3' miRNA: 3'- -GCuuCGCU---UCACCgACGCGuuGCUUG- -5' |
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| 17005 | 3' | -52.4 | NC_004333.2 | + | 19603 | 0.73 | 0.397375 |
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Target: 5'- aCGAcGCGAacaaGGUcacGGCgaGCGCGGCGAGCg -3' miRNA: 3'- -GCUuCGCU----UCA---CCGa-CGCGUUGCUUG- -5' |
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| 17005 | 3' | -52.4 | NC_004333.2 | + | 42081 | 0.74 | 0.378742 |
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Target: 5'- aCGAggauAGCGucGU-GCUGCGCGACGAGg -3' miRNA: 3'- -GCU----UCGCuuCAcCGACGCGUUGCUUg -5' |
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| 17005 | 3' | -52.4 | NC_004333.2 | + | 13886 | 0.74 | 0.369649 |
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Target: 5'- ---cGCGAAGUcggccggcacgGGCUGCGCGGCGGc- -3' miRNA: 3'- gcuuCGCUUCA-----------CCGACGCGUUGCUug -5' |
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| 17005 | 3' | -52.4 | NC_004333.2 | + | 9293 | 0.76 | 0.272474 |
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Target: 5'- gCGAAGCGgcGcGGgUGCGCgGACGGGCg -3' miRNA: 3'- -GCUUCGCuuCaCCgACGCG-UUGCUUG- -5' |
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| 17005 | 3' | -52.4 | NC_004333.2 | + | 42712 | 1.11 | 0.001092 |
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Target: 5'- cCGAAGCGAAGUGGCUGCGCAACGAACa -3' miRNA: 3'- -GCUUCGCUUCACCGACGCGUUGCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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