Results 21 - 33 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17009 | 5' | -55.8 | NC_004333.2 | + | 43326 | 0.67 | 0.527203 |
Target: 5'- -gCAUCGGCacGGCgcGCGCGUACGGUc -3' miRNA: 3'- uaGUAGCUGc-UCGa-CGCGCGUGUCAa -5' |
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17009 | 5' | -55.8 | NC_004333.2 | + | 30996 | 0.67 | 0.527203 |
Target: 5'- --uGUUG-CGGGCUGCuCGCGCGGUa -3' miRNA: 3'- uagUAGCuGCUCGACGcGCGUGUCAa -5' |
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17009 | 5' | -55.8 | NC_004333.2 | + | 36212 | 0.67 | 0.54918 |
Target: 5'- gAUCGUCGAC--GCUGCGUGCAaGGc- -3' miRNA: 3'- -UAGUAGCUGcuCGACGCGCGUgUCaa -5' |
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17009 | 5' | -55.8 | NC_004333.2 | + | 21892 | 0.67 | 0.54918 |
Target: 5'- -gCAUCGACGGcGCgGUGCGCGCc--- -3' miRNA: 3'- uaGUAGCUGCU-CGaCGCGCGUGucaa -5' |
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17009 | 5' | -55.8 | NC_004333.2 | + | 40046 | 0.66 | 0.560276 |
Target: 5'- uGUCGUCGACGGGCUcGuCGgGCAUc--- -3' miRNA: 3'- -UAGUAGCUGCUCGA-C-GCgCGUGucaa -5' |
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17009 | 5' | -55.8 | NC_004333.2 | + | 25116 | 0.66 | 0.560276 |
Target: 5'- -gCA-CGGCGAGCacggcgGCGCGCACGu-- -3' miRNA: 3'- uaGUaGCUGCUCGa-----CGCGCGUGUcaa -5' |
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17009 | 5' | -55.8 | NC_004333.2 | + | 20752 | 0.66 | 0.560276 |
Target: 5'- cAUCGcCGACGcGGCUGCGCGC-CuGa- -3' miRNA: 3'- -UAGUaGCUGC-UCGACGCGCGuGuCaa -5' |
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17009 | 5' | -55.8 | NC_004333.2 | + | 27438 | 0.66 | 0.571434 |
Target: 5'- -gCGUCGGCGAuguccgGCaGCGCGCGCGc-- -3' miRNA: 3'- uaGUAGCUGCU------CGaCGCGCGUGUcaa -5' |
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17009 | 5' | -55.8 | NC_004333.2 | + | 44389 | 0.66 | 0.582643 |
Target: 5'- ---cUCGGCG-GC-GCGUGCGCGGUa -3' miRNA: 3'- uaguAGCUGCuCGaCGCGCGUGUCAa -5' |
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17009 | 5' | -55.8 | NC_004333.2 | + | 32320 | 0.66 | 0.593897 |
Target: 5'- --gGUCGgucACGAGC-GCGUGCACGGc- -3' miRNA: 3'- uagUAGC---UGCUCGaCGCGCGUGUCaa -5' |
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17009 | 5' | -55.8 | NC_004333.2 | + | 26515 | 0.66 | 0.605185 |
Target: 5'- uAUCuUCGAgcaaauggcCGAuCUGCGCGCGCAGc- -3' miRNA: 3'- -UAGuAGCU---------GCUcGACGCGCGUGUCaa -5' |
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17009 | 5' | -55.8 | NC_004333.2 | + | 10510 | 0.66 | 0.605185 |
Target: 5'- ----cCGACGAGCgggucggGCGCGCGCu--- -3' miRNA: 3'- uaguaGCUGCUCGa------CGCGCGUGucaa -5' |
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17009 | 5' | -55.8 | NC_004333.2 | + | 8866 | 0.66 | 0.605185 |
Target: 5'- -gUAUCG-CG-GCUGCG-GCGCGGUUa -3' miRNA: 3'- uaGUAGCuGCuCGACGCgCGUGUCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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