Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17194 | 5' | -61.5 | NC_004333.2 | + | 41550 | 0.66 | 0.38714 |
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Target: 5'- aCGCGcUCCGacuuCUCGAGCUcgccauggaucaCGAcGGCCg -3' miRNA: 3'- aGCGC-AGGCcu--GAGCUCGG------------GCU-CCGG- -5' |
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| 17194 | 5' | -61.5 | NC_004333.2 | + | 45650 | 0.66 | 0.395785 |
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Target: 5'- cUCGCGaCCGGGCUggucgagcgccaUGAGCaCCGAGuaUu -3' miRNA: 3'- -AGCGCaGGCCUGA------------GCUCG-GGCUCcgG- -5' |
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| 17194 | 5' | -61.5 | NC_004333.2 | + | 13011 | 0.66 | 0.404553 |
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Target: 5'- uUCGCG-CCGGGCaucaaCGccuGCgCGAGGUCc -3' miRNA: 3'- -AGCGCaGGCCUGa----GCu--CGgGCUCCGG- -5' |
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| 17194 | 5' | -61.5 | NC_004333.2 | + | 27741 | 0.66 | 0.422453 |
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Target: 5'- gCGCGaaUCUGGuCggccgcCGAGCCCGucAGGCg -3' miRNA: 3'- aGCGC--AGGCCuGa-----GCUCGGGC--UCCGg -5' |
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| 17194 | 5' | -61.5 | NC_004333.2 | + | 10147 | 0.66 | 0.422453 |
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Target: 5'- cCGCGaUCC-GAC-CGAGCUCG-GGCa -3' miRNA: 3'- aGCGC-AGGcCUGaGCUCGGGCuCCGg -5' |
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| 17194 | 5' | -61.5 | NC_004333.2 | + | 25524 | 0.66 | 0.422453 |
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Target: 5'- gCGCGUCuUGGuACgCGccGCUCGAGGUCa -3' miRNA: 3'- aGCGCAG-GCC-UGaGCu-CGGGCUCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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