Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17368 | 5' | -53.8 | NC_004367.1 | + | 8656 | 0.66 | 0.951573 |
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Target: 5'- gAGAAACguaAUACa-ACAGAGGCaUACCAa -3' miRNA: 3'- -UCUUUGg--UAUGggUGUCUCCG-GUGGU- -5' |
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| 17368 | 5' | -53.8 | NC_004367.1 | + | 64766 | 0.66 | 0.951573 |
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Target: 5'- uAGAugaCAUuCCCcUGGAGGCCACUAu -3' miRNA: 3'- -UCUuugGUAuGGGuGUCUCCGGUGGU- -5' |
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| 17368 | 5' | -53.8 | NC_004367.1 | + | 126586 | 0.66 | 0.951573 |
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Target: 5'- cAGAGACCuUACCCACu---GUCACUAg -3' miRNA: 3'- -UCUUUGGuAUGGGUGucucCGGUGGU- -5' |
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| 17368 | 5' | -53.8 | NC_004367.1 | + | 91342 | 0.66 | 0.955638 |
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Target: 5'- uAGAGAUUu--CCU--GGAGGCCACCGc -3' miRNA: 3'- -UCUUUGGuauGGGugUCUCCGGUGGU- -5' |
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| 17368 | 5' | -53.8 | NC_004367.1 | + | 101531 | 0.66 | 0.955638 |
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Target: 5'- aGGAAuuccaGCCAUGCCCAUcauGGCUugUAu -3' miRNA: 3'- -UCUU-----UGGUAUGGGUGucuCCGGugGU- -5' |
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| 17368 | 5' | -53.8 | NC_004367.1 | + | 73930 | 0.66 | 0.955638 |
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Target: 5'- uGGGAAaUAUGCCUAUaAGAGGCCuCCu -3' miRNA: 3'- -UCUUUgGUAUGGGUG-UCUCCGGuGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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