Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17368 | 5' | -53.8 | NC_004367.1 | + | 126586 | 0.66 | 0.951573 |
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Target: 5'- cAGAGACCuUACCCACu---GUCACUAg -3' miRNA: 3'- -UCUUUGGuAUGGGUGucucCGGUGGU- -5' |
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| 17368 | 5' | -53.8 | NC_004367.1 | + | 126688 | 0.67 | 0.927561 |
|
Target: 5'- uAGGGACUAgGCCgGC--GGGCCGCCu -3' miRNA: 3'- -UCUUUGGUaUGGgUGucUCCGGUGGu -5' |
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| 17368 | 5' | -53.8 | NC_004367.1 | + | 127110 | 0.67 | 0.927561 |
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Target: 5'- uAGGGACUAgGCCgGC--GGGCCGCCu -3' miRNA: 3'- -UCUUUGGUaUGGgUGucUCCGGUGGu -5' |
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| 17368 | 5' | -53.8 | NC_004367.1 | + | 129597 | 0.67 | 0.927561 |
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Target: 5'- uAGGGACUAgGCCgGC--GGGCCGCCu -3' miRNA: 3'- -UCUUUGGUaUGGgUGucUCCGGUGGu -5' |
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| 17368 | 5' | -53.8 | NC_004367.1 | + | 130019 | 0.67 | 0.927561 |
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Target: 5'- uAGGGACUAgGCCgGC--GGGCCGCCu -3' miRNA: 3'- -UCUUUGGUaUGGgUGucUCCGGUGGu -5' |
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| 17368 | 5' | -53.8 | NC_004367.1 | + | 135131 | 0.69 | 0.850623 |
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Target: 5'- uGGAAACCGUGagugcguccugagcCCCGCA-AGcGCCACCu -3' miRNA: 3'- -UCUUUGGUAU--------------GGGUGUcUC-CGGUGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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