Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17439 | 3' | -50.7 | NC_004453.1 | + | 21365 | 0.67 | 0.801532 |
Target: 5'- gGGAGAgagcgaccaUGGCGGAaaagcUUGGCCGAa- -3' miRNA: 3'- aCCUCU---------ACUGCUUgau--AACCGGCUgc -5' |
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17439 | 3' | -50.7 | NC_004453.1 | + | 14123 | 0.67 | 0.78067 |
Target: 5'- --cGGcgGACGAAaUGaUGGCCGACGc -3' miRNA: 3'- accUCuaCUGCUUgAUaACCGGCUGC- -5' |
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17439 | 3' | -50.7 | NC_004453.1 | + | 1024 | 0.67 | 0.78067 |
Target: 5'- uUGGAGAagaUGAgcacaaCGGACUGUcGGCUuGACGu -3' miRNA: 3'- -ACCUCU---ACU------GCUUGAUAaCCGG-CUGC- -5' |
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17439 | 3' | -50.7 | NC_004453.1 | + | 2450 | 0.67 | 0.769969 |
Target: 5'- gGGAGAaGugGGACU----GCCGGCa -3' miRNA: 3'- aCCUCUaCugCUUGAuaacCGGCUGc -5' |
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17439 | 3' | -50.7 | NC_004453.1 | + | 16472 | 0.68 | 0.725688 |
Target: 5'- gGGAGAaGacaauaccggaGCGGACauuUUGGCCGACc -3' miRNA: 3'- aCCUCUaC-----------UGCUUGau-AACCGGCUGc -5' |
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17439 | 3' | -50.7 | NC_004453.1 | + | 17857 | 0.68 | 0.714319 |
Target: 5'- cGGcGAUGGgguaGAGCaUGUUGGCCGcCGa -3' miRNA: 3'- aCCuCUACUg---CUUG-AUAACCGGCuGC- -5' |
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17439 | 3' | -50.7 | NC_004453.1 | + | 3613 | 0.79 | 0.206265 |
Target: 5'- ------cGACGAACUGUUGGCCGACc -3' miRNA: 3'- accucuaCUGCUUGAUAACCGGCUGc -5' |
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17439 | 3' | -50.7 | NC_004453.1 | + | 6760 | 1.09 | 0.001752 |
Target: 5'- cUGGAGAUGACGAACUAUUGGCCGACGc -3' miRNA: 3'- -ACCUCUACUGCUUGAUAACCGGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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