miRNA: 3'- agagUGGCCGaAUGgGCCGgGCCGCG- -5'
miRNA: 3'- agaGUGGCCgaa-----UGggCCGGGCCGCG- -5'
miRNA: 3'- agaGUgGCCGA------------AUGGGC-----CGGGCCGCG- -5'
miRNA: 3'- aGAgUGG----CCGa----AUGG-GCCGGG-CCGCG- -5'
miRNA: 3'- -AGAGUGGCCGAAUGGGCCGGGCCGCG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17453 | 5' | -61.8 | NC_004453.1 | + | 20183 | 0.66 | 0.279949 |
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Target: 5'- ---gGCCGGC-UGCgUGGUgUGGCGCc -3' miRNA: 3'- agagUGGCCGaAUGgGCCGgGCCGCG- -5' |
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| 17453 | 5' | -61.8 | NC_004453.1 | + | 26742 | 0.68 | 0.216805 |
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Target: 5'- --aCACCGGauugauggGCgaGGCUCGGCGCu -3' miRNA: 3'- agaGUGGCCgaa-----UGggCCGGGCCGCG- -5' |
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| 17453 | 5' | -61.8 | NC_004453.1 | + | 19522 | 0.69 | 0.180197 |
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Target: 5'- --aCAgCGGCUuucguuuuccgcUGCCCGaagccGCCUGGCGCc -3' miRNA: 3'- agaGUgGCCGA------------AUGGGC-----CGGGCCGCG- -5' |
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| 17453 | 5' | -61.8 | NC_004453.1 | + | 20081 | 0.7 | 0.137429 |
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Target: 5'- gCUgGCCgaggGGCcccagUACCaCGGCCCaGGCGCc -3' miRNA: 3'- aGAgUGG----CCGa----AUGG-GCCGGG-CCGCG- -5' |
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| 17453 | 5' | -61.8 | NC_004453.1 | + | 14384 | 1.1 | 0.000106 |
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Target: 5'- uUCUCACCGGCUUACCCGGCCCGGCGCc -3' miRNA: 3'- -AGAGUGGCCGAAUGGGCCGGGCCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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