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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17460 | 5' | -58.4 | NC_004453.1 | + | 18920 | 0.66 | 0.38167 |
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Target: 5'- -uGG-CCAGCCAGugCacu-CCGCCGg -3' miRNA: 3'- ucCCgGGUCGGUCugGaaauGGUGGC- -5' |
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| 17460 | 5' | -58.4 | NC_004453.1 | + | 12776 | 0.66 | 0.371758 |
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Target: 5'- aGGGGCgCAGCUaccacgucgggcaAGACCc-UACgGCCGa -3' miRNA: 3'- -UCCCGgGUCGG-------------UCUGGaaAUGgUGGC- -5' |
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| 17460 | 5' | -58.4 | NC_004453.1 | + | 14653 | 0.67 | 0.32651 |
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Target: 5'- uGGGGUCCGGCCAgcucgcucaugcccGAcuacgugcugcauCCUUcuaucgugccUACCGCCGg -3' miRNA: 3'- -UCCCGGGUCGGU--------------CU-------------GGAA----------AUGGUGGC- -5' |
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| 17460 | 5' | -58.4 | NC_004453.1 | + | 10796 | 0.7 | 0.225894 |
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Target: 5'- cGGGUcgcaaacugugaaccUCAGCCGGGCCUUUGaCACCc -3' miRNA: 3'- uCCCG---------------GGUCGGUCUGGAAAUgGUGGc -5' |
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| 17460 | 5' | -58.4 | NC_004453.1 | + | 20272 | 1.08 | 0.000294 |
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Target: 5'- aAGGGCCCAGCCAGACCUUUACCACCGc -3' miRNA: 3'- -UCCCGGGUCGGUCUGGAAAUGGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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