Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17469 | 3' | -47.8 | NC_004456.1 | + | 20178 | 0.66 | 0.95719 |
Target: 5'- cCUGACCaguuCAGCCGGuGCGGGCAGc--- -3' miRNA: 3'- aGACUGG----GUUGGUU-UGUUCGUCuauu -5' |
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17469 | 3' | -47.8 | NC_004456.1 | + | 20875 | 0.66 | 0.95719 |
Target: 5'- aCUGACCCGGaggCAAACGGcCAGAg-- -3' miRNA: 3'- aGACUGGGUUg--GUUUGUUcGUCUauu -5' |
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17469 | 3' | -47.8 | NC_004456.1 | + | 11551 | 0.66 | 0.942481 |
Target: 5'- uUCUGGCUgCAAUCGcGCAAGCAGc--- -3' miRNA: 3'- -AGACUGG-GUUGGUuUGUUCGUCuauu -5' |
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17469 | 3' | -47.8 | NC_004456.1 | + | 2875 | 0.67 | 0.933436 |
Target: 5'- cCUGGCCaugcaggcaaagcaGGCCAGaACAGGCGGAc-- -3' miRNA: 3'- aGACUGGg-------------UUGGUU-UGUUCGUCUauu -5' |
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17469 | 3' | -47.8 | NC_004456.1 | + | 4486 | 0.67 | 0.924827 |
Target: 5'- cCUGGCucgCCAGCUGGACAaAGCAGGa-- -3' miRNA: 3'- aGACUG---GGUUGGUUUGU-UCGUCUauu -5' |
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17469 | 3' | -47.8 | NC_004456.1 | + | 6603 | 0.68 | 0.896654 |
Target: 5'- cCUGugUCuGCCAGGCGAGCAa---- -3' miRNA: 3'- aGACugGGuUGGUUUGUUCGUcuauu -5' |
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17469 | 3' | -47.8 | NC_004456.1 | + | 35485 | 0.69 | 0.825482 |
Target: 5'- --aGACCC-GCC-AGCAAGCGGAg-- -3' miRNA: 3'- agaCUGGGuUGGuUUGUUCGUCUauu -5' |
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17469 | 3' | -47.8 | NC_004456.1 | + | 6707 | 1.07 | 0.004592 |
Target: 5'- gUCUGACCCAACCAAACAAGCAGAUAAc -3' miRNA: 3'- -AGACUGGGUUGGUUUGUUCGUCUAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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