Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17473 | 3' | -46.8 | NC_004456.1 | + | 3088 | 0.7 | 0.902696 |
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Target: 5'- ----aCCACUGAGGCcccGGCAguacguagcauuuGAGCCCCu -3' miRNA: 3'- guucaGGUGAUUUUG---CUGU-------------UUCGGGG- -5' |
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| 17473 | 3' | -46.8 | NC_004456.1 | + | 19414 | 0.7 | 0.901224 |
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Target: 5'- ---cUCCGCUGGcuACGGCAAAauccauaaaccaacGCCCCa -3' miRNA: 3'- guucAGGUGAUUu-UGCUGUUU--------------CGGGG- -5' |
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| 17473 | 3' | -46.8 | NC_004456.1 | + | 12581 | 0.7 | 0.880131 |
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Target: 5'- cCGAuGUCgugaGCUGGAAUGACuu-GCCCCu -3' miRNA: 3'- -GUU-CAGg---UGAUUUUGCUGuuuCGGGG- -5' |
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| 17473 | 3' | -46.8 | NC_004456.1 | + | 31186 | 0.72 | 0.795195 |
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Target: 5'- gAAGUCCACc-GGACGGCAcAGGCCa- -3' miRNA: 3'- gUUCAGGUGauUUUGCUGU-UUCGGgg -5' |
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| 17473 | 3' | -46.8 | NC_004456.1 | + | 22332 | 0.74 | 0.717615 |
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Target: 5'- gCAGGUcaaaaCCACgucccauGCGGCGAGGCUCCa -3' miRNA: 3'- -GUUCA-----GGUGauuu---UGCUGUUUCGGGG- -5' |
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| 17473 | 3' | -46.8 | NC_004456.1 | + | 27612 | 0.74 | 0.694344 |
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Target: 5'- uGAGggCCACcgauAACGGCAGAGCCgCCc -3' miRNA: 3'- gUUCa-GGUGauu-UUGCUGUUUCGG-GG- -5' |
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| 17473 | 3' | -46.8 | NC_004456.1 | + | 16102 | 0.76 | 0.564173 |
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Target: 5'- aUAGGUCUGCUAGGGCuucguACAgcAAGCCCCg -3' miRNA: 3'- -GUUCAGGUGAUUUUGc----UGU--UUCGGGG- -5' |
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| 17473 | 3' | -46.8 | NC_004456.1 | + | 12521 | 1.15 | 0.002382 |
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Target: 5'- aCAAGUCCACUAAAACGACAAAGCCCCg -3' miRNA: 3'- -GUUCAGGUGAUUUUGCUGUUUCGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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