Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17473 | 3' | -46.8 | NC_004456.1 | + | 12521 | 1.15 | 0.002382 |
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Target: 5'- aCAAGUCCACUAAAACGACAAAGCCCCg -3' miRNA: 3'- -GUUCAGGUGAUUUUGCUGUUUCGGGG- -5' |
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| 17473 | 3' | -46.8 | NC_004456.1 | + | 12404 | 0.68 | 0.940917 |
|
Target: 5'- aAAG-CCGcCUAGAGCGGuuuuuugcguuucCAGGGCCCUg -3' miRNA: 3'- gUUCaGGU-GAUUUUGCU-------------GUUUCGGGG- -5' |
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| 17473 | 3' | -46.8 | NC_004456.1 | + | 11926 | 0.68 | 0.951091 |
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Target: 5'- gGGGUCCGCUGguuccuuggugugGAGCGAau-GGCCaCUa -3' miRNA: 3'- gUUCAGGUGAU-------------UUUGCUguuUCGG-GG- -5' |
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| 17473 | 3' | -46.8 | NC_004456.1 | + | 9326 | 0.66 | 0.984754 |
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Target: 5'- -uGGUgCGCUAcuGCGAaAAAGCCUUg -3' miRNA: 3'- guUCAgGUGAUuuUGCUgUUUCGGGG- -5' |
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| 17473 | 3' | -46.8 | NC_004456.1 | + | 8490 | 0.66 | 0.986712 |
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Target: 5'- -cAGUCCGCUGucuguuGCGaaaGCAgcGCCaCCa -3' miRNA: 3'- guUCAGGUGAUuu----UGC---UGUuuCGG-GG- -5' |
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| 17473 | 3' | -46.8 | NC_004456.1 | + | 6364 | 0.67 | 0.968116 |
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Target: 5'- gCAAGUCUACcaua--GACAgcGCCCUu -3' miRNA: 3'- -GUUCAGGUGauuuugCUGUuuCGGGG- -5' |
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| 17473 | 3' | -46.8 | NC_004456.1 | + | 5490 | 0.66 | 0.977554 |
|
Target: 5'- aAAG-CCGCaAAAGCGGCcuucacagucauAGAGUCCCc -3' miRNA: 3'- gUUCaGGUGaUUUUGCUG------------UUUCGGGG- -5' |
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| 17473 | 3' | -46.8 | NC_004456.1 | + | 3088 | 0.7 | 0.902696 |
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Target: 5'- ----aCCACUGAGGCcccGGCAguacguagcauuuGAGCCCCu -3' miRNA: 3'- guucaGGUGAUUUUG---CUGU-------------UUCGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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