Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17473 | 5' | -49.4 | NC_004456.1 | + | 12487 | 1.15 | 0.001218 |
Target: 5'- gUCAGGGCCCUGGAAACGCAAAAAACCg -3' miRNA: 3'- -AGUCCCGGGACCUUUGCGUUUUUUGG- -5' |
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17473 | 5' | -49.4 | NC_004456.1 | + | 31912 | 0.73 | 0.551395 |
Target: 5'- ---cGGCaCCUGGAAAUcugGCGAAAAACCc -3' miRNA: 3'- agucCCG-GGACCUUUG---CGUUUUUUGG- -5' |
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17473 | 5' | -49.4 | NC_004456.1 | + | 21339 | 0.73 | 0.562867 |
Target: 5'- -gAGGGCCaauuuaaUGGAAugGCAAcccuuuuGACCa -3' miRNA: 3'- agUCCCGGg------ACCUUugCGUUuu-----UUGG- -5' |
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17473 | 5' | -49.4 | NC_004456.1 | + | 2176 | 0.71 | 0.667729 |
Target: 5'- --uGGGCCCacUGGAAAgGCGGAuGAUCc -3' miRNA: 3'- aguCCCGGG--ACCUUUgCGUUUuUUGG- -5' |
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17473 | 5' | -49.4 | NC_004456.1 | + | 7025 | 0.71 | 0.713867 |
Target: 5'- --cGGGCgCUGGcgcuACGCuuAAAACCu -3' miRNA: 3'- aguCCCGgGACCuu--UGCGuuUUUUGG- -5' |
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17473 | 5' | -49.4 | NC_004456.1 | + | 38727 | 0.69 | 0.820914 |
Target: 5'- cCuGGGCgCUGGAAAUGau---GACCa -3' miRNA: 3'- aGuCCCGgGACCUUUGCguuuuUUGG- -5' |
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17473 | 5' | -49.4 | NC_004456.1 | + | 35934 | 0.67 | 0.875262 |
Target: 5'- gCGGaGGCCCUGGcaagAGACuuAGcAAACCa -3' miRNA: 3'- aGUC-CCGGGACC----UUUGcgUUuUUUGG- -5' |
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17473 | 5' | -49.4 | NC_004456.1 | + | 33429 | 0.67 | 0.875262 |
Target: 5'- cCGGGGCUCagucGAAGgcgaaGCAGGAGGCCa -3' miRNA: 3'- aGUCCCGGGac--CUUUg----CGUUUUUUGG- -5' |
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17473 | 5' | -49.4 | NC_004456.1 | + | 41942 | 0.67 | 0.883377 |
Target: 5'- cUCAGGGCCUU-GAAcCGUAucagguugcGAGACCu -3' miRNA: 3'- -AGUCCCGGGAcCUUuGCGUu--------UUUUGG- -5' |
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17473 | 5' | -49.4 | NC_004456.1 | + | 14224 | 0.67 | 0.891202 |
Target: 5'- uUCAcGGGCCUUGGcuuCGguAAu-GCCa -3' miRNA: 3'- -AGU-CCCGGGACCuuuGCguUUuuUGG- -5' |
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17473 | 5' | -49.4 | NC_004456.1 | + | 35447 | 0.67 | 0.89799 |
Target: 5'- gUCAGGGCCUaaucuugagcaauUGGccGAgGCugAAAAGACCc -3' miRNA: 3'- -AGUCCCGGG-------------ACCu-UUgCG--UUUUUUGG- -5' |
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17473 | 5' | -49.4 | NC_004456.1 | + | 39572 | 0.67 | 0.908757 |
Target: 5'- -gAGGGCUccagauuaacccaaaCUGaGAAAaucaGCAAGAAGCCc -3' miRNA: 3'- agUCCCGG---------------GAC-CUUUg---CGUUUUUUGG- -5' |
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17473 | 5' | -49.4 | NC_004456.1 | + | 35261 | 0.66 | 0.919479 |
Target: 5'- gUCuGGuGCCuuuucccuCUGGGAcGCGCuuAAAACCg -3' miRNA: 3'- -AGuCC-CGG--------GACCUU-UGCGuuUUUUGG- -5' |
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17473 | 5' | -49.4 | NC_004456.1 | + | 28202 | 0.66 | 0.931759 |
Target: 5'- gCGGcGGCCuCUGGuguCGCAAcuauggcGACCg -3' miRNA: 3'- aGUC-CCGG-GACCuuuGCGUUuu-----UUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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