Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17474 | 3' | -51.8 | NC_004456.1 | + | 23174 | 0.66 | 0.795913 |
Target: 5'- cAGCGG--GCGCAagggcgguuuacUGAGCuGCUGcGCCa -3' miRNA: 3'- uUUGCCuaCGUGU------------AUUCGuCGAC-CGG- -5' |
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17474 | 3' | -51.8 | NC_004456.1 | + | 4270 | 0.67 | 0.742065 |
Target: 5'- gAAACug--GCACAgcagAacaagaccaccAGCAGCUGGCCa -3' miRNA: 3'- -UUUGccuaCGUGUa---U-----------UCGUCGACCGG- -5' |
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17474 | 3' | -51.8 | NC_004456.1 | + | 41363 | 0.67 | 0.730863 |
Target: 5'- -cAUGGAgggcaaaaUGCACGUAgcuaAGCAaauGCUGGCa -3' miRNA: 3'- uuUGCCU--------ACGUGUAU----UCGU---CGACCGg -5' |
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17474 | 3' | -51.8 | NC_004456.1 | + | 3196 | 0.67 | 0.708134 |
Target: 5'- -cACGGAUGCcuGCAUcGGCAccuGCUGaGCa -3' miRNA: 3'- uuUGCCUACG--UGUAuUCGU---CGAC-CGg -5' |
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17474 | 3' | -51.8 | NC_004456.1 | + | 14074 | 0.67 | 0.708134 |
Target: 5'- uGGCGGAU-CAUuucAAGCAuCUGGCCg -3' miRNA: 3'- uUUGCCUAcGUGua-UUCGUcGACCGG- -5' |
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17474 | 3' | -51.8 | NC_004456.1 | + | 12951 | 0.68 | 0.685067 |
Target: 5'- aAAACGGA-GUGCAUAAGCgccaucuaaAGaaGGCCu -3' miRNA: 3'- -UUUGCCUaCGUGUAUUCG---------UCgaCCGG- -5' |
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17474 | 3' | -51.8 | NC_004456.1 | + | 35868 | 0.68 | 0.650068 |
Target: 5'- cAACuGAUGCAU--GAGCAGCaGGCg -3' miRNA: 3'- uUUGcCUACGUGuaUUCGUCGaCCGg -5' |
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17474 | 3' | -51.8 | NC_004456.1 | + | 33145 | 0.69 | 0.62662 |
Target: 5'- -uGCGGcaGCAgAaAGGCAGgUGGCCg -3' miRNA: 3'- uuUGCCuaCGUgUaUUCGUCgACCGG- -5' |
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17474 | 3' | -51.8 | NC_004456.1 | + | 19248 | 0.7 | 0.533917 |
Target: 5'- ---aGGAUGCcauagcccuuaGCAaugcUAAGCAGCUGGUa -3' miRNA: 3'- uuugCCUACG-----------UGU----AUUCGUCGACCGg -5' |
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17474 | 3' | -51.8 | NC_004456.1 | + | 16793 | 0.7 | 0.522618 |
Target: 5'- -uGCGGAcaagGCACAUGaaaguAGC-GCUGGUCu -3' miRNA: 3'- uuUGCCUa---CGUGUAU-----UCGuCGACCGG- -5' |
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17474 | 3' | -51.8 | NC_004456.1 | + | 36192 | 0.71 | 0.489312 |
Target: 5'- --gUGGAUGC-CAUGGccGCucaGGCUGGCCu -3' miRNA: 3'- uuuGCCUACGuGUAUU--CG---UCGACCGG- -5' |
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17474 | 3' | -51.8 | NC_004456.1 | + | 32752 | 0.71 | 0.467669 |
Target: 5'- uGAGCuGAUGCGCu--GGCgagAGUUGGCCa -3' miRNA: 3'- -UUUGcCUACGUGuauUCG---UCGACCGG- -5' |
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17474 | 3' | -51.8 | NC_004456.1 | + | 15279 | 1.04 | 0.002702 |
Target: 5'- uAAACGGAUGCACAUAAGCAGCUGGCg -3' miRNA: 3'- -UUUGCCUACGUGUAUUCGUCGACCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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