Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
17475 | 5' | -52 | NC_004456.1 | + | 32680 | 0.66 | 0.817742 |
Target: 5'- -aGACGAaGCGUggGCCGauauuGCGGCGg -3' miRNA: 3'- uaUUGCUaCGCG--UGGCaguu-UGCCGC- -5' |
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17475 | 5' | -52 | NC_004456.1 | + | 34663 | 0.67 | 0.766813 |
Target: 5'- uUAGcCGGUGCGcCGCUGUUAu-UGGCGa -3' miRNA: 3'- uAUU-GCUACGC-GUGGCAGUuuGCCGC- -5' |
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17475 | 5' | -52 | NC_004456.1 | + | 19729 | 0.67 | 0.745293 |
Target: 5'- gAUGAUGAUGCGgGCCagugauuuGUCGgccauugcGAUGGCGu -3' miRNA: 3'- -UAUUGCUACGCgUGG--------CAGU--------UUGCCGC- -5' |
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17475 | 5' | -52 | NC_004456.1 | + | 15439 | 0.7 | 0.609256 |
Target: 5'- aGUAACGAUGCggGCugCGUCGAcagcaaccucuACGGg- -3' miRNA: 3'- -UAUUGCUACG--CGugGCAGUU-----------UGCCgc -5' |
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17475 | 5' | -52 | NC_004456.1 | + | 37267 | 0.7 | 0.586347 |
Target: 5'- -aAGCGAgGCGCACUGgUgAAACGGuCGg -3' miRNA: 3'- uaUUGCUaCGCGUGGC-AgUUUGCC-GC- -5' |
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17475 | 5' | -52 | NC_004456.1 | + | 16537 | 0.7 | 0.563605 |
Target: 5'- uUGACGGUGCGCAUCGUUAucuuugaugaGAUGaGUGc -3' miRNA: 3'- uAUUGCUACGCGUGGCAGU----------UUGC-CGC- -5' |
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17475 | 5' | -52 | NC_004456.1 | + | 16599 | 1.08 | 0.001804 |
Target: 5'- gAUAACGAUGCGCACCGUCAAACGGCGa -3' miRNA: 3'- -UAUUGCUACGCGUGGCAGUUUGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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