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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 17482 | 5' | -54.7 | NC_004456.1 | + | 12223 | 0.66 | 0.624013 |
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Target: 5'- -gGAUAaaauagcccgcuUGC-CCGCCAUuucaGCCUCUGCc -3' miRNA: 3'- caCUAU------------ACGaGGUGGUGc---CGGAGACG- -5' |
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| 17482 | 5' | -54.7 | NC_004456.1 | + | 24838 | 0.66 | 0.63536 |
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Target: 5'- ---cUcgGCUCCcaacggGCCAaaGCCUCUGCc -3' miRNA: 3'- cacuAuaCGAGG------UGGUgcCGGAGACG- -5' |
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| 17482 | 5' | -54.7 | NC_004456.1 | + | 15179 | 0.67 | 0.578805 |
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Target: 5'- uUGGUGUGCauaagguuggCCGCUccaguaGCGGCCagCUGCu -3' miRNA: 3'- cACUAUACGa---------GGUGG------UGCCGGa-GACG- -5' |
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| 17482 | 5' | -54.7 | NC_004456.1 | + | 10553 | 0.67 | 0.612674 |
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Target: 5'- uUGuuuUAUGgUCCACC-UGGCCUUccggUGCa -3' miRNA: 3'- cACu--AUACgAGGUGGuGCCGGAG----ACG- -5' |
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| 17482 | 5' | -54.7 | NC_004456.1 | + | 10140 | 0.67 | 0.612674 |
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Target: 5'- uUGuuuUAUGgUCCACC-UGGCCUUccggUGCa -3' miRNA: 3'- cACu--AUACgAGGUGGuGCCGGAG----ACG- -5' |
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| 17482 | 5' | -54.7 | NC_004456.1 | + | 26084 | 0.68 | 0.523403 |
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Target: 5'- uUGAUAUGCUCCGgCCAgCGGUCg---- -3' miRNA: 3'- cACUAUACGAGGU-GGU-GCCGGagacg -5' |
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| 17482 | 5' | -54.7 | NC_004456.1 | + | 22930 | 0.68 | 0.534339 |
|
Target: 5'- uUGAUGgguaGCUUUACuCACGGCaCUCUcGCu -3' miRNA: 3'- cACUAUa---CGAGGUG-GUGCCG-GAGA-CG- -5' |
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| 17482 | 5' | -54.7 | NC_004456.1 | + | 26681 | 0.72 | 0.313382 |
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Target: 5'- -aGAUAaGCUCUGCCAgccCGGCCUCaGUg -3' miRNA: 3'- caCUAUaCGAGGUGGU---GCCGGAGaCG- -5' |
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| 17482 | 5' | -54.7 | NC_004456.1 | + | 26309 | 0.73 | 0.290183 |
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Target: 5'- gGUGAccUGCUCCAgCAUGGCUUCgGUu -3' miRNA: 3'- -CACUauACGAGGUgGUGCCGGAGaCG- -5' |
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| 17482 | 5' | -54.7 | NC_004456.1 | + | 24553 | 1.12 | 0.000438 |
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Target: 5'- gGUGAUAUGCUCCACCACGGCCUCUGCa -3' miRNA: 3'- -CACUAUACGAGGUGGUGCCGGAGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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