Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17485 | 3' | -53.9 | NC_004456.1 | + | 20609 | 0.68 | 0.54233 |
Target: 5'- aAGCGGUgAaaagCGCAAAGGCcgaAGCCaCACa -3' miRNA: 3'- -UCGUCGgUaa--GCGUUUCUG---UCGG-GUG- -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 18283 | 0.68 | 0.553595 |
Target: 5'- uGGCGGCCAccgUUgGCAggacaucgGAGACuauGGCCCGg -3' miRNA: 3'- -UCGUCGGU---AAgCGU--------UUCUG---UCGGGUg -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 33485 | 0.68 | 0.554726 |
Target: 5'- cGGCAgGCCAaaaagcuggagagaaUCGCAGAGAUagaAGCCCGu -3' miRNA: 3'- -UCGU-CGGUa--------------AGCGUUUCUG---UCGGGUg -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 27722 | 0.76 | 0.173335 |
Target: 5'- cGCAGCCAUgaaGCGAAGugGGaaaCCGCu -3' miRNA: 3'- uCGUCGGUAag-CGUUUCugUCg--GGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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