Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17604 | 3' | -46.3 | NC_004466.2 | + | 39932 | 0.66 | 0.992452 |
Target: 5'- cGGUAACACC----GGCGGCucccuugGGugCGGu -3' miRNA: 3'- -CCAUUGUGGuauuCUGUUG-------UCugGCC- -5' |
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17604 | 3' | -46.3 | NC_004466.2 | + | 88 | 0.66 | 0.992214 |
Target: 5'- aGGacAgACCAUAAcGACGACacuaacgcggaaagGGGCCGGc -3' miRNA: 3'- -CCauUgUGGUAUU-CUGUUG--------------UCUGGCC- -5' |
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17604 | 3' | -46.3 | NC_004466.2 | + | 2252 | 0.66 | 0.991332 |
Target: 5'- gGGUAuuCGCCucAAGGCAGCccaggauauccuGGACCGa -3' miRNA: 3'- -CCAUu-GUGGuaUUCUGUUG------------UCUGGCc -5' |
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17604 | 3' | -46.3 | NC_004466.2 | + | 33590 | 0.66 | 0.991332 |
Target: 5'- cGU-ACACCGUAAGAUcACGGuuCCGu -3' miRNA: 3'- cCAuUGUGGUAUUCUGuUGUCu-GGCc -5' |
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17604 | 3' | -46.3 | NC_004466.2 | + | 44671 | 0.66 | 0.989939 |
Target: 5'- cGUAuCGCCGUGAucCAGC-GACUGGa -3' miRNA: 3'- cCAUuGUGGUAUUcuGUUGuCUGGCC- -5' |
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17604 | 3' | -46.3 | NC_004466.2 | + | 40700 | 0.67 | 0.988376 |
Target: 5'- gGGUccgcGACACC-UGGGGCAaGCuGuGCCGGg -3' miRNA: 3'- -CCA----UUGUGGuAUUCUGU-UGuC-UGGCC- -5' |
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17604 | 3' | -46.3 | NC_004466.2 | + | 29957 | 0.67 | 0.987351 |
Target: 5'- uGGUAACACCGggcuaguguucaccAAGuACAACccuGACaCGGg -3' miRNA: 3'- -CCAUUGUGGUa-------------UUC-UGUUGu--CUG-GCC- -5' |
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17604 | 3' | -46.3 | NC_004466.2 | + | 22353 | 0.67 | 0.98663 |
Target: 5'- uGGUGACGCUGUAGGcgGCGAagaGGAUUGa -3' miRNA: 3'- -CCAUUGUGGUAUUC--UGUUg--UCUGGCc -5' |
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17604 | 3' | -46.3 | NC_004466.2 | + | 13985 | 0.67 | 0.98663 |
Target: 5'- uGGUAACGCCAUGcaGGuuGAU--GCUGGa -3' miRNA: 3'- -CCAUUGUGGUAU--UCugUUGucUGGCC- -5' |
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17604 | 3' | -46.3 | NC_004466.2 | + | 32368 | 0.67 | 0.984691 |
Target: 5'- aGGUAGCGCCcugGAGGCAuauaccCAGGCa-- -3' miRNA: 3'- -CCAUUGUGGua-UUCUGUu-----GUCUGgcc -5' |
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17604 | 3' | -46.3 | NC_004466.2 | + | 14353 | 0.68 | 0.980178 |
Target: 5'- --aGAgGCCAUuAGaACAACAGACCc- -3' miRNA: 3'- ccaUUgUGGUAuUC-UGUUGUCUGGcc -5' |
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17604 | 3' | -46.3 | NC_004466.2 | + | 26282 | 0.68 | 0.977581 |
Target: 5'- uGGUGAUcCUagAUGAGAagguaCGACGGGCUGGa -3' miRNA: 3'- -CCAUUGuGG--UAUUCU-----GUUGUCUGGCC- -5' |
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17604 | 3' | -46.3 | NC_004466.2 | + | 4498 | 0.68 | 0.971649 |
Target: 5'- aGG-GACGuCCAaGAGACcgUAGACUGGa -3' miRNA: 3'- -CCaUUGU-GGUaUUCUGuuGUCUGGCC- -5' |
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17604 | 3' | -46.3 | NC_004466.2 | + | 29515 | 0.68 | 0.971649 |
Target: 5'- aGUGuC-CCAUAGGACGGuCAGGCCa- -3' miRNA: 3'- cCAUuGuGGUAUUCUGUU-GUCUGGcc -5' |
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17604 | 3' | -46.3 | NC_004466.2 | + | 37414 | 0.68 | 0.967233 |
Target: 5'- uGGUGAUGCCGU-GGugGaauugcucgaaggaGCuGGCCGGa -3' miRNA: 3'- -CCAUUGUGGUAuUCugU--------------UGuCUGGCC- -5' |
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17604 | 3' | -46.3 | NC_004466.2 | + | 11338 | 0.69 | 0.964663 |
Target: 5'- uGGUAGgGuCCGUuGGAuccCAAgAGGCCGGg -3' miRNA: 3'- -CCAUUgU-GGUAuUCU---GUUgUCUGGCC- -5' |
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17604 | 3' | -46.3 | NC_004466.2 | + | 35786 | 0.69 | 0.956552 |
Target: 5'- -uUGACgGCCAaGAGACGAC-GACCGa -3' miRNA: 3'- ccAUUG-UGGUaUUCUGUUGuCUGGCc -5' |
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17604 | 3' | -46.3 | NC_004466.2 | + | 5534 | 0.69 | 0.953891 |
Target: 5'- cGGggAAguCUAUGGGAUgcuugaccgccuugaGGCAGACCGGg -3' miRNA: 3'- -CCa-UUguGGUAUUCUG---------------UUGUCUGGCC- -5' |
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17604 | 3' | -46.3 | NC_004466.2 | + | 22114 | 0.69 | 0.952056 |
Target: 5'- cGGaacCACCGUAAGGCc---GACCGGa -3' miRNA: 3'- -CCauuGUGGUAUUCUGuuguCUGGCC- -5' |
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17604 | 3' | -46.3 | NC_004466.2 | + | 1024 | 0.7 | 0.93101 |
Target: 5'- --gGAUACCAUAAGGC--UAGuCCGGg -3' miRNA: 3'- ccaUUGUGGUAUUCUGuuGUCuGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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