Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17969 | 5' | -58.8 | NC_004665.1 | + | 34997 | 0.66 | 0.442261 |
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Target: 5'- aCCGGCgCUCAGuuucuuGGCCAUGUCGa- -3' miRNA: 3'- -GGCUG-GGGUCucuu--CCGGUGCAGCca -5' |
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| 17969 | 5' | -58.8 | NC_004665.1 | + | 32813 | 0.67 | 0.413768 |
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Target: 5'- gCGGCCCCGGuGAcguuuAGGCCACcagaaGUCa-- -3' miRNA: 3'- gGCUGGGGUCuCU-----UCCGGUG-----CAGcca -5' |
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| 17969 | 5' | -58.8 | NC_004665.1 | + | 22075 | 0.68 | 0.351888 |
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Target: 5'- gUCGGCCaCAGuGAugAGGCgCAgGUCGGUg -3' miRNA: 3'- -GGCUGGgGUCuCU--UCCG-GUgCAGCCA- -5' |
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| 17969 | 5' | -58.8 | NC_004665.1 | + | 503 | 0.68 | 0.318813 |
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Target: 5'- aUCGACaCCAGAGccuauaAGGGCCACaagcuggGUCGGg -3' miRNA: 3'- -GGCUGgGGUCUC------UUCCGGUG-------CAGCCa -5' |
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| 17969 | 5' | -58.8 | NC_004665.1 | + | 31430 | 0.69 | 0.304296 |
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Target: 5'- aCCGGCaCCCGGAaccugcuccaGAAGGCCGCuuagUGGa -3' miRNA: 3'- -GGCUG-GGGUCU----------CUUCCGGUGca--GCCa -5' |
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| 17969 | 5' | -58.8 | NC_004665.1 | + | 3646 | 0.7 | 0.268537 |
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Target: 5'- aCCGAUCCCuGAGAuGGgCACGcUGGa -3' miRNA: 3'- -GGCUGGGGuCUCUuCCgGUGCaGCCa -5' |
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| 17969 | 5' | -58.8 | NC_004665.1 | + | 18187 | 0.7 | 0.248754 |
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Target: 5'- gCGACUCCAGAGAguucAGGUCGCcgaGGUa -3' miRNA: 3'- gGCUGGGGUCUCU----UCCGGUGcagCCA- -5' |
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| 17969 | 5' | -58.8 | NC_004665.1 | + | 27445 | 0.73 | 0.162842 |
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Target: 5'- -aGACCUCcuguuAGAGAGGGCCACGgcccccUCGGa -3' miRNA: 3'- ggCUGGGG-----UCUCUUCCGGUGC------AGCCa -5' |
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| 17969 | 5' | -58.8 | NC_004665.1 | + | 4454 | 1.08 | 0.000377 |
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Target: 5'- gCCGACCCCAGAGAAGGCCACGUCGGUc -3' miRNA: 3'- -GGCUGGGGUCUCUUCCGGUGCAGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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