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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17972 | 3' | -57.3 | NC_004665.1 | + | 34673 | 0.66 | 0.454576 |
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Target: 5'- ------aGCCCGAuauuGCGACUGCGCagaUCAg -3' miRNA: 3'- acagaaaCGGGCU----CGCUGGCGCG---AGU- -5' |
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| 17972 | 3' | -57.3 | NC_004665.1 | + | 19842 | 0.68 | 0.360325 |
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Target: 5'- aGUCgagGCCCGAGUGGgCGC-UUCGa -3' miRNA: 3'- aCAGaaaCGGGCUCGCUgGCGcGAGU- -5' |
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| 17972 | 3' | -57.3 | NC_004665.1 | + | 11044 | 0.69 | 0.302934 |
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Target: 5'- gGUCgagGCCgaagguugcCGGGUGAUCGUGCUCGa -3' miRNA: 3'- aCAGaaaCGG---------GCUCGCUGGCGCGAGU- -5' |
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| 17972 | 3' | -57.3 | NC_004665.1 | + | 28872 | 0.7 | 0.287883 |
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Target: 5'- uUGUCUUgaaGCCUuccugcuuGUGGCUGCGCUCAc -3' miRNA: 3'- -ACAGAAa--CGGGcu------CGCUGGCGCGAGU- -5' |
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| 17972 | 3' | -57.3 | NC_004665.1 | + | 26969 | 0.73 | 0.178024 |
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Target: 5'- cGUCguagagaGUCCGAGCGAUC-CGCUCAa -3' miRNA: 3'- aCAGaaa----CGGGCUCGCUGGcGCGAGU- -5' |
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| 17972 | 3' | -57.3 | NC_004665.1 | + | 6131 | 1.09 | 0.000377 |
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Target: 5'- gUGUCUUUGCCCGAGCGACCGCGCUCAg -3' miRNA: 3'- -ACAGAAACGGGCUCGCUGGCGCGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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