Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17974 | 5' | -53.6 | NC_004665.1 | + | 6750 | 0.66 | 0.683292 |
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Target: 5'- uGCCGAUcuggGCUgAGUCCAucGAGAccGCu -3' miRNA: 3'- gCGGCUA----CGGgUCAGGUuaCUCUa-CG- -5' |
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| 17974 | 5' | -53.6 | NC_004665.1 | + | 13358 | 0.66 | 0.671918 |
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Target: 5'- cCGacaaCGGUGCUCcgaaGGUUgaCGAUGAGGUGCu -3' miRNA: 3'- -GCg---GCUACGGG----UCAG--GUUACUCUACG- -5' |
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| 17974 | 5' | -53.6 | NC_004665.1 | + | 32191 | 0.67 | 0.626132 |
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Target: 5'- aGCCGAUGCCUgagcuuGGUUCAAaGcAGAgccUGCc -3' miRNA: 3'- gCGGCUACGGG------UCAGGUUaC-UCU---ACG- -5' |
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| 17974 | 5' | -53.6 | NC_004665.1 | + | 1560 | 0.67 | 0.603227 |
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Target: 5'- gGCCcucGCCCAG-CgCAAUGGGAUGa -3' miRNA: 3'- gCGGcuaCGGGUCaG-GUUACUCUACg -5' |
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| 17974 | 5' | -53.6 | NC_004665.1 | + | 31521 | 0.69 | 0.535464 |
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Target: 5'- uGCCGGUcauguaGCCCAGcUCUuggGAGcgGCg -3' miRNA: 3'- gCGGCUA------CGGGUC-AGGuuaCUCuaCG- -5' |
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| 17974 | 5' | -53.6 | NC_004665.1 | + | 9526 | 0.69 | 0.513432 |
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Target: 5'- aGCUcaugGUCCAGUCCcgaagGGGAUGCa -3' miRNA: 3'- gCGGcua-CGGGUCAGGuua--CUCUACG- -5' |
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| 17974 | 5' | -53.6 | NC_004665.1 | + | 32709 | 0.71 | 0.38145 |
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Target: 5'- uGCUGGUGCCCAGaCCAAUGccaacGGAa-- -3' miRNA: 3'- gCGGCUACGGGUCaGGUUAC-----UCUacg -5' |
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| 17974 | 5' | -53.6 | NC_004665.1 | + | 25388 | 0.77 | 0.176789 |
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Target: 5'- aGCCGAUGagaGGUCCAAUGAGAUcauccGCa -3' miRNA: 3'- gCGGCUACgggUCAGGUUACUCUA-----CG- -5' |
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| 17974 | 5' | -53.6 | NC_004665.1 | + | 9024 | 1.12 | 0.000538 |
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Target: 5'- gCGCCGAUGCCCAGUCCAAUGAGAUGCu -3' miRNA: 3'- -GCGGCUACGGGUCAGGUUACUCUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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