Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17978 | 3' | -55.2 | NC_004665.1 | + | 24697 | 0.66 | 0.524051 |
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Target: 5'- cAUAGUGccGCCCAcgagGGCGACaGCGGC-CAu -3' miRNA: 3'- -UAUCAC--CGGGU----UCGUUG-CGUCGaGUu -5' |
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| 17978 | 3' | -55.2 | NC_004665.1 | + | 26182 | 0.66 | 0.479708 |
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Target: 5'- ---aUGGCCguAGUAAUGCccAGCUCGAa -3' miRNA: 3'- uaucACCGGguUCGUUGCG--UCGAGUU- -5' |
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| 17978 | 3' | -55.2 | NC_004665.1 | + | 17572 | 0.67 | 0.427012 |
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Target: 5'- -gAGUGGCa--GGCAAaGCAGCUCGu -3' miRNA: 3'- uaUCACCGgguUCGUUgCGUCGAGUu -5' |
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| 17978 | 3' | -55.2 | NC_004665.1 | + | 32289 | 0.68 | 0.377905 |
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Target: 5'- uUGGUGGCCgAAGUGGCaGCA-CUCGAa -3' miRNA: 3'- uAUCACCGGgUUCGUUG-CGUcGAGUU- -5' |
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| 17978 | 3' | -55.2 | NC_004665.1 | + | 6133 | 0.69 | 0.315817 |
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Target: 5'- -gGGUGucuuuGCCCGAGCGAcCGC-GCUCAGa -3' miRNA: 3'- uaUCAC-----CGGGUUCGUU-GCGuCGAGUU- -5' |
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| 17978 | 3' | -55.2 | NC_004665.1 | + | 10321 | 0.72 | 0.221606 |
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Target: 5'- -cAGUGGCCCAGuuGCAGgGCGGCa--- -3' miRNA: 3'- uaUCACCGGGUU--CGUUgCGUCGaguu -5' |
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| 17978 | 3' | -55.2 | NC_004665.1 | + | 13768 | 1.04 | 0.000822 |
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Target: 5'- cAUAGUGGCCCAAGCAACGCAGCUCAAg -3' miRNA: 3'- -UAUCACCGGGUUCGUUGCGUCGAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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