miRNA: 3'- -CGUAGGUgUGUUGGU-CUGGUAGuGCc -5'
miRNA: 3'- -CGUAGgU-GUGUUGGU-CUGgUAGU-GCC- -5'
miRNA: 3'- -CGUAGGUGUGUUGgucu----GGUAgUGCC- -5'
miRNA: 3'- cGUAGGU-GUgUuGGUCUGGuAGUGCc -5'
miRNA: 3'- -CGUAGGUGUguUGGucuGGU--AGUGCC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18002 | 3' | -50.9 | NC_004665.1 | + | 3326 | 0.66 | 0.84153 |
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Target: 5'- cGCAUUCAaGCGACCGuGACUGUC-CGa -3' miRNA: 3'- -CGUAGGUgUGUUGGU-CUGGUAGuGCc -5' |
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| 18002 | 3' | -50.9 | NC_004665.1 | + | 14562 | 0.66 | 0.84153 |
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Target: 5'- gGUGUCgGuCGCAACCAcGAUgGUCAuCGGg -3' miRNA: 3'- -CGUAGgU-GUGUUGGU-CUGgUAGU-GCC- -5' |
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| 18002 | 3' | -50.9 | NC_004665.1 | + | 12996 | 0.66 | 0.850586 |
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Target: 5'- aGCGUCCAUGCAGCagcgugcuCCAU-ACGGa -3' miRNA: 3'- -CGUAGGUGUGUUGgucu----GGUAgUGCC- -5' |
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| 18002 | 3' | -50.9 | NC_004665.1 | + | 22463 | 0.66 | 0.859388 |
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Target: 5'- uCAUCUAuCAgGuCCAGACCuUCACGa -3' miRNA: 3'- cGUAGGU-GUgUuGGUCUGGuAGUGCc -5' |
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| 18002 | 3' | -50.9 | NC_004665.1 | + | 35495 | 0.66 | 0.859388 |
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Target: 5'- cGUAUCCACG--GCCuu-CCAgcUCGCGGu -3' miRNA: 3'- -CGUAGGUGUguUGGucuGGU--AGUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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