miRNA: 3'- -CGUAGGUGuGUUGGUCuGGuAGUgCC- -5'
miRNA: 3'- cGUAGG----UGUgUUGGUCUGGUaGUGCC- -5'
miRNA: 3'- -CGUaGGUGUGUUgGUCUGGUaGUGCC- -5'
miRNA: 3'- cguAGGUGUGUU-GGUCUGGUAgUGCC- -5'
miRNA: 3'- -CGUAGGUGUGUUGGUCUGGUAGUGCC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18002 | 3' | -50.9 | NC_004665.1 | + | 26218 | 0.7 | 0.648671 |
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Target: 5'- aGCAUCCGC-CAGCCGG-UC-UCAgGGu -3' miRNA: 3'- -CGUAGGUGuGUUGGUCuGGuAGUgCC- -5' |
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| 18002 | 3' | -50.9 | NC_004665.1 | + | 25855 | 0.72 | 0.522975 |
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Target: 5'- uCGUCCagcaACGgAGCCAGAUCGcCACGGg -3' miRNA: 3'- cGUAGG----UGUgUUGGUCUGGUaGUGCC- -5' |
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| 18002 | 3' | -50.9 | NC_004665.1 | + | 14836 | 0.74 | 0.407713 |
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Target: 5'- uGCAgUCGCACGAaCGGGCCAgCACGGa -3' miRNA: 3'- -CGUaGGUGUGUUgGUCUGGUaGUGCC- -5' |
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| 18002 | 3' | -50.9 | NC_004665.1 | + | 15600 | 0.78 | 0.237104 |
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Target: 5'- --uUCCACAUGAuCCAGACCAUgAUGGg -3' miRNA: 3'- cguAGGUGUGUU-GGUCUGGUAgUGCC- -5' |
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| 18002 | 3' | -50.9 | NC_004665.1 | + | 3894 | 1.13 | 0.001004 |
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Target: 5'- aGCAUCCACACAACCAGACCAUCACGGu -3' miRNA: 3'- -CGUAGGUGUGUUGGUCUGGUAGUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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