Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18010 | 5' | -55.9 | NC_004665.1 | + | 28674 | 0.66 | 0.545266 |
Target: 5'- -cGCCAcgGCgaagcuGGuCUGCCUaaccgugacguuGAGCUGCCu -3' miRNA: 3'- gaCGGUaaCG------CC-GAUGGG------------UUCGACGG- -5' |
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18010 | 5' | -55.9 | NC_004665.1 | + | 33226 | 0.66 | 0.534174 |
Target: 5'- -gGCCAgcgUUGUGGa-ACCCGAguGCUGCa -3' miRNA: 3'- gaCGGU---AACGCCgaUGGGUU--CGACGg -5' |
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18010 | 5' | -55.9 | NC_004665.1 | + | 28536 | 0.66 | 0.523164 |
Target: 5'- -gGCCAcuaUGCGGCUGCUgcugUAGGUggUGCUg -3' miRNA: 3'- gaCGGUa--ACGCCGAUGG----GUUCG--ACGG- -5' |
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18010 | 5' | -55.9 | NC_004665.1 | + | 13499 | 0.66 | 0.501414 |
Target: 5'- aUGCCGaUGC-GCUucuggaucaGCUCAauGGCUGCCu -3' miRNA: 3'- gACGGUaACGcCGA---------UGGGU--UCGACGG- -5' |
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18010 | 5' | -55.9 | NC_004665.1 | + | 28342 | 0.67 | 0.490689 |
Target: 5'- uUGCCucUGgGaGCUugUCAcGGCUGCCc -3' miRNA: 3'- gACGGuaACgC-CGAugGGU-UCGACGG- -5' |
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18010 | 5' | -55.9 | NC_004665.1 | + | 18909 | 0.67 | 0.469568 |
Target: 5'- uUGCCucgggacUUGCGGUaGCCUGAGCaGCg -3' miRNA: 3'- gACGGu------AACGCCGaUGGGUUCGaCGg -5' |
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18010 | 5' | -55.9 | NC_004665.1 | + | 2275 | 0.67 | 0.46644 |
Target: 5'- -aGaCCcUUGCGGCguccuuucgguacacCCCAgcGGCUGCCu -3' miRNA: 3'- gaC-GGuAACGCCGau-------------GGGU--UCGACGG- -5' |
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18010 | 5' | -55.9 | NC_004665.1 | + | 26673 | 0.67 | 0.459182 |
Target: 5'- gUGCCuaUGCGGCUgACCCA-GC-GaCCa -3' miRNA: 3'- gACGGuaACGCCGA-UGGGUuCGaC-GG- -5' |
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18010 | 5' | -55.9 | NC_004665.1 | + | 18343 | 0.68 | 0.399607 |
Target: 5'- aUGCCAUUGaGGCacGCCUGAGCUacGUCu -3' miRNA: 3'- gACGGUAACgCCGa-UGGGUUCGA--CGG- -5' |
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18010 | 5' | -55.9 | NC_004665.1 | + | 22086 | 0.68 | 0.390166 |
Target: 5'- cCUGaCCcaUGCGGcCUACCCAcGGCUGg- -3' miRNA: 3'- -GAC-GGuaACGCC-GAUGGGU-UCGACgg -5' |
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18010 | 5' | -55.9 | NC_004665.1 | + | 28925 | 0.69 | 0.353894 |
Target: 5'- -aGCCcgUGaGGCUGCUcgCAAGCUGgCg -3' miRNA: 3'- gaCGGuaACgCCGAUGG--GUUCGACgG- -5' |
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18010 | 5' | -55.9 | NC_004665.1 | + | 7703 | 0.71 | 0.281277 |
Target: 5'- cCUGCCAUcGCuGGCgguUCCAAGCUGa- -3' miRNA: 3'- -GACGGUAaCG-CCGau-GGGUUCGACgg -5' |
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18010 | 5' | -55.9 | NC_004665.1 | + | 28449 | 0.71 | 0.259845 |
Target: 5'- gCUGCCcgUGUGG--GCaagCAGGCUGCCu -3' miRNA: 3'- -GACGGuaACGCCgaUGg--GUUCGACGG- -5' |
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18010 | 5' | -55.9 | NC_004665.1 | + | 36252 | 0.72 | 0.233365 |
Target: 5'- aUGaCCAgcUUGUGGCUGCCCAguacaGGCU-CCa -3' miRNA: 3'- gAC-GGU--AACGCCGAUGGGU-----UCGAcGG- -5' |
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18010 | 5' | -55.9 | NC_004665.1 | + | 15355 | 0.72 | 0.233365 |
Target: 5'- -aGCCAgu-CGGCUACCCGcacuuCUGCCa -3' miRNA: 3'- gaCGGUaacGCCGAUGGGUuc---GACGG- -5' |
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18010 | 5' | -55.9 | NC_004665.1 | + | 32152 | 0.73 | 0.209191 |
Target: 5'- gCUGCCAgUGCGaGUcaauccGCCCAGucuGCUGCCa -3' miRNA: 3'- -GACGGUaACGC-CGa-----UGGGUU---CGACGG- -5' |
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18010 | 5' | -55.9 | NC_004665.1 | + | 11648 | 1.11 | 0.000278 |
Target: 5'- gCUGCCAUUGCGGCUACCCAAGCUGCCu -3' miRNA: 3'- -GACGGUAACGCCGAUGGGUUCGACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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