Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18052 | 5' | -56.3 | NC_004680.1 | + | 16937 | 0.66 | 0.710729 |
Target: 5'- uCCCCA---GUCGCGGgGCAgGUGCCGg -3' miRNA: 3'- -GGGGUgccUAGCGUCaUGUaCGCGGU- -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 42934 | 0.66 | 0.710729 |
Target: 5'- aCCCgcaaaGCGGAUCuGCGGUAUuUGCGaCGu -3' miRNA: 3'- gGGG-----UGCCUAG-CGUCAUGuACGCgGU- -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 15281 | 0.66 | 0.710729 |
Target: 5'- -gUCGCGGAUaccgCGCAGUuCAgcgGUGCCGg -3' miRNA: 3'- ggGGUGCCUA----GCGUCAuGUa--CGCGGU- -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 59311 | 0.66 | 0.700118 |
Target: 5'- gCCCGCGuucGAUCaGguGUucgaccCAUGCGCCu -3' miRNA: 3'- gGGGUGC---CUAG-CguCAu-----GUACGCGGu -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 21494 | 0.66 | 0.700118 |
Target: 5'- cCCCCAuucgcCGGAUCGUcGU-CGUGCGaaaCGg -3' miRNA: 3'- -GGGGU-----GCCUAGCGuCAuGUACGCg--GU- -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 39640 | 0.66 | 0.667943 |
Target: 5'- gCCgCACGGAgcgcCGCAGgga--GCGCCGc -3' miRNA: 3'- -GGgGUGCCUa---GCGUCauguaCGCGGU- -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 39844 | 0.66 | 0.667943 |
Target: 5'- gCCgCACGGAgcgcCGCAGgga--GCGCCGc -3' miRNA: 3'- -GGgGUGCCUa---GCGUCauguaCGCGGU- -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 36003 | 0.66 | 0.657139 |
Target: 5'- cCCCgGCGGccagauucgAUCGCGG-ACAagGUGCCGc -3' miRNA: 3'- -GGGgUGCC---------UAGCGUCaUGUa-CGCGGU- -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 4209 | 0.67 | 0.646313 |
Target: 5'- gUUCGCGGAcaUCGCAGaccgcgGCGgacGCGCCGu -3' miRNA: 3'- gGGGUGCCU--AGCGUCa-----UGUa--CGCGGU- -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 21314 | 0.67 | 0.646313 |
Target: 5'- -gCCACGGuguUCGCGGUggugGCGUGgGaCCGc -3' miRNA: 3'- ggGGUGCCu--AGCGUCA----UGUACgC-GGU- -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 8105 | 0.67 | 0.61813 |
Target: 5'- aCUCCAgccggugaaucagaGGAUCaGCAGUACcgaGCGCCAc -3' miRNA: 3'- -GGGGUg-------------CCUAG-CGUCAUGua-CGCGGU- -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 23113 | 0.67 | 0.602977 |
Target: 5'- -gCCACGGGUgGUGG-AUAUGCGCgGg -3' miRNA: 3'- ggGGUGCCUAgCGUCaUGUACGCGgU- -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 16672 | 0.68 | 0.596496 |
Target: 5'- cCUCCaguugcggccgcaggGCGGuAUCGguGUugGUGUGCCc -3' miRNA: 3'- -GGGG---------------UGCC-UAGCguCAugUACGCGGu -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 44863 | 0.68 | 0.581419 |
Target: 5'- uCCCCACGGuggCGgAGUACcgaaaacaaGCGCUg -3' miRNA: 3'- -GGGGUGCCua-GCgUCAUGua-------CGCGGu -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 29089 | 0.68 | 0.581419 |
Target: 5'- gCCCGCGccgaguguggcGAUCGCGGUuugggcgaACGUGCggGCCGc -3' miRNA: 3'- gGGGUGC-----------CUAGCGUCA--------UGUACG--CGGU- -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 59065 | 0.68 | 0.569627 |
Target: 5'- gCCCCAgCGGugugUGguGUguuggggGCAUGUGCCGg -3' miRNA: 3'- -GGGGU-GCCua--GCguCA-------UGUACGCGGU- -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 47833 | 0.69 | 0.507635 |
Target: 5'- aUCCCGCcGGAUCGCAcGUGCA-GCaUCAa -3' miRNA: 3'- -GGGGUG-CCUAGCGU-CAUGUaCGcGGU- -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 38110 | 0.69 | 0.507635 |
Target: 5'- -aUCGCGGAU-GUAGUcCGUGUGCCAa -3' miRNA: 3'- ggGGUGCCUAgCGUCAuGUACGCGGU- -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 27741 | 0.69 | 0.481216 |
Target: 5'- gUCCCACGGGaaccagucaccaacaUCGguGUGgGUGCcCCAc -3' miRNA: 3'- -GGGGUGCCU---------------AGCguCAUgUACGcGGU- -5' |
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18052 | 5' | -56.3 | NC_004680.1 | + | 47660 | 0.7 | 0.438111 |
Target: 5'- -aCCACGGAUCGUAG-GCG-GCGCa- -3' miRNA: 3'- ggGGUGCCUAGCGUCaUGUaCGCGgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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