Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18056 | 5' | -57.7 | NC_004680.1 | + | 20749 | 0.77 | 0.121849 |
Target: 5'- gGCCGCcgAuCGGGCCUGuUCCGCCGc -3' miRNA: 3'- gUGGCGuaUcGCCUGGACuAGGCGGU- -5' |
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18056 | 5' | -57.7 | NC_004680.1 | + | 25907 | 0.66 | 0.585275 |
Target: 5'- gGCCGaacuggAGCGGGCCaacgcaacuAUCCGCCGc -3' miRNA: 3'- gUGGCgua---UCGCCUGGac-------UAGGCGGU- -5' |
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18056 | 5' | -57.7 | NC_004680.1 | + | 44275 | 0.66 | 0.583127 |
Target: 5'- gCACCGCucucgcggacaagGUAGCGGucauagacacagaACgCgGAUCCGCCu -3' miRNA: 3'- -GUGGCG-------------UAUCGCC-------------UG-GaCUAGGCGGu -5' |
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18056 | 5' | -57.7 | NC_004680.1 | + | 19097 | 0.67 | 0.541636 |
Target: 5'- gGCCGUGUGGCGGAUUUGccgaaccAUCCGaCGu -3' miRNA: 3'- gUGGCGUAUCGCCUGGAC-------UAGGCgGU- -5' |
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18056 | 5' | -57.7 | NC_004680.1 | + | 26815 | 0.67 | 0.521772 |
Target: 5'- gACCGCAagUGGUGGugCc---CCGCCAa -3' miRNA: 3'- gUGGCGU--AUCGCCugGacuaGGCGGU- -5' |
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18056 | 5' | -57.7 | NC_004680.1 | + | 23202 | 0.67 | 0.512461 |
Target: 5'- gGCgGCAggcaccuauguggagGGCGGcAUCUGGUCgGCCAg -3' miRNA: 3'- gUGgCGUa--------------UCGCC-UGGACUAGgCGGU- -5' |
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18056 | 5' | -57.7 | NC_004680.1 | + | 27710 | 0.69 | 0.422816 |
Target: 5'- gGCgGC-UGGCGGcaagaccgaACCUGAaCCGCCGa -3' miRNA: 3'- gUGgCGuAUCGCC---------UGGACUaGGCGGU- -5' |
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18056 | 5' | -57.7 | NC_004680.1 | + | 52936 | 0.69 | 0.413542 |
Target: 5'- -gUCGCAUAGCGGcggcauUCUcGUCCGCCGc -3' miRNA: 3'- guGGCGUAUCGCCu-----GGAcUAGGCGGU- -5' |
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18056 | 5' | -57.7 | NC_004680.1 | + | 1837 | 0.7 | 0.344071 |
Target: 5'- uCGCCGCGUgGGCGGGCC-GGUUC-CCGg -3' miRNA: 3'- -GUGGCGUA-UCGCCUGGaCUAGGcGGU- -5' |
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18056 | 5' | -57.7 | NC_004680.1 | + | 41757 | 0.72 | 0.283398 |
Target: 5'- cCGCCGCAUcuGCuGGACCUGccCCGuCCAa -3' miRNA: 3'- -GUGGCGUAu-CG-CCUGGACuaGGC-GGU- -5' |
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18056 | 5' | -57.7 | NC_004680.1 | + | 2673 | 0.74 | 0.198044 |
Target: 5'- aGCCGU--GGCGGAUCaGGUCCGCUAu -3' miRNA: 3'- gUGGCGuaUCGCCUGGaCUAGGCGGU- -5' |
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18056 | 5' | -57.7 | NC_004680.1 | + | 25501 | 0.8 | 0.073647 |
Target: 5'- aCACCGCGUGGCGGggcucaagGCCUGAgaaaccgaccCCGCCAc -3' miRNA: 3'- -GUGGCGUAUCGCC--------UGGACUa---------GGCGGU- -5' |
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18056 | 5' | -57.7 | NC_004680.1 | + | 27296 | 0.66 | 0.606826 |
Target: 5'- uGCCGCu--GCGGGCgCUGuuGUUgGCCGa -3' miRNA: 3'- gUGGCGuauCGCCUG-GAC--UAGgCGGU- -5' |
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18056 | 5' | -57.7 | NC_004680.1 | + | 25911 | 0.66 | 0.585275 |
Target: 5'- uCGCCGUgGUGGCGG-CCg---CCGCCGc -3' miRNA: 3'- -GUGGCG-UAUCGCCuGGacuaGGCGGU- -5' |
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18056 | 5' | -57.7 | NC_004680.1 | + | 53031 | 0.66 | 0.585275 |
Target: 5'- aGCCuCGcGGCGGACgaGAaugCCGCCGc -3' miRNA: 3'- gUGGcGUaUCGCCUGgaCUa--GGCGGU- -5' |
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18056 | 5' | -57.7 | NC_004680.1 | + | 12698 | 0.67 | 0.553252 |
Target: 5'- cCAUCGCGUccAGCGccaugccgccGGCCUGcaCCGCCAu -3' miRNA: 3'- -GUGGCGUA--UCGC----------CUGGACuaGGCGGU- -5' |
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18056 | 5' | -57.7 | NC_004680.1 | + | 12786 | 0.67 | 0.521772 |
Target: 5'- gCGCCGauc-GCGGcgACCUGG-CCGCCGu -3' miRNA: 3'- -GUGGCguauCGCC--UGGACUaGGCGGU- -5' |
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18056 | 5' | -57.7 | NC_004680.1 | + | 28169 | 0.69 | 0.432214 |
Target: 5'- uGCCGCGUuguauCGGGCCUGcauuUCCGCg- -3' miRNA: 3'- gUGGCGUAuc---GCCUGGACu---AGGCGgu -5' |
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18056 | 5' | -57.7 | NC_004680.1 | + | 51746 | 0.69 | 0.413542 |
Target: 5'- uCACCgagGUAUGcGCGGACCUucccgGAgCCGCCAg -3' miRNA: 3'- -GUGG---CGUAU-CGCCUGGA-----CUaGGCGGU- -5' |
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18056 | 5' | -57.7 | NC_004680.1 | + | 20324 | 0.69 | 0.413542 |
Target: 5'- uGCCGC---GCGGACCggUGAUCUGCa- -3' miRNA: 3'- gUGGCGuauCGCCUGG--ACUAGGCGgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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