Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18063 | 5' | -55 | NC_004680.1 | + | 8880 | 0.66 | 0.770735 |
Target: 5'- uGAcCGC-GCCGGGACGg-GUCUCCGc -3' miRNA: 3'- gCUuGCGuCGGUCUUGCggUAGAGGC- -5' |
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18063 | 5' | -55 | NC_004680.1 | + | 9309 | 0.65 | 0.780706 |
Target: 5'- gCGAACaGCAcgcGCCAccGCGCCAUUgaCCGg -3' miRNA: 3'- -GCUUG-CGU---CGGUcuUGCGGUAGa-GGC- -5' |
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18063 | 5' | -55 | NC_004680.1 | + | 12729 | 0.65 | 0.780706 |
Target: 5'- cCGGucuGCGCGGCCuGAccaGCGCCGggugCCa -3' miRNA: 3'- -GCU---UGCGUCGGuCU---UGCGGUaga-GGc -5' |
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18063 | 5' | -55 | NC_004680.1 | + | 5629 | 1.09 | 0.001277 |
Target: 5'- aCGAACGCAGCCAGAACGCCAUCUCCGc -3' miRNA: 3'- -GCUUGCGUCGGUCUUGCGGUAGAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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