miRNA: 3'- -GCUUgC-GUCGGUCuUGCGGuag-AGGC- -5'
miRNA: 3'- gcuuGCGUCGGUC--UUGCGGua--GAGGC- -5'
miRNA: 3'- gCUUGCGUCGGUCUUGCGgua-----GAgGc -5'
miRNA: 3'- gCuuGCGUCG-----GUCUUGCGGUaGAGGc -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18063 | 5' | -55 | NC_004680.1 | + | 47337 | 0.68 | 0.621373 |
|
Target: 5'- gCGAGgGaCAGCCGG-ACGCCcuuaUCCGg -3' miRNA: 3'- -GCUUgC-GUCGGUCuUGCGGuag-AGGC- -5' |
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| 18063 | 5' | -55 | NC_004680.1 | + | 47915 | 0.67 | 0.686817 |
|
Target: 5'- ---cCGCAGCCAGccGGCGCUccaaCUCCGc -3' miRNA: 3'- gcuuGCGUCGGUC--UUGCGGua--GAGGC- -5' |
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| 18063 | 5' | -55 | NC_004680.1 | + | 57202 | 0.69 | 0.577793 |
|
Target: 5'- aGAAUGCAGCCGGGACGUagguguggCUgCa -3' miRNA: 3'- gCUUGCGUCGGUCUUGCGgua-----GAgGc -5' |
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| 18063 | 5' | -55 | NC_004680.1 | + | 57869 | 0.67 | 0.675978 |
|
Target: 5'- gGucCGCAGCagacgCGGAACGCUAgCUCCa -3' miRNA: 3'- gCuuGCGUCG-----GUCUUGCGGUaGAGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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