Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18065 | 5' | -47.6 | NC_004680.1 | + | 27333 | 0.66 | 0.989669 |
Target: 5'- -cCGCCuuccugggugUGGAuCAGGAaCAUGGUGGGCu -3' miRNA: 3'- caGUGG----------ACUU-GUCUUaGUGCCAUUCG- -5' |
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18065 | 5' | -47.6 | NC_004680.1 | + | 2447 | 0.66 | 0.988096 |
Target: 5'- aUCAuCCcGAACGGGAUaACGGUuGGUg -3' miRNA: 3'- cAGU-GGaCUUGUCUUAgUGCCAuUCG- -5' |
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18065 | 5' | -47.6 | NC_004680.1 | + | 12782 | 0.66 | 0.988096 |
Target: 5'- -aCAaCUGGAucacCAGGAUCGCGGgcGGUu -3' miRNA: 3'- caGUgGACUU----GUCUUAGUGCCauUCG- -5' |
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18065 | 5' | -47.6 | NC_004680.1 | + | 47693 | 0.66 | 0.986343 |
Target: 5'- -aCGCCUcGACGGuggCGCGGUucGGCu -3' miRNA: 3'- caGUGGAcUUGUCuuaGUGCCAu-UCG- -5' |
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18065 | 5' | -47.6 | NC_004680.1 | + | 3398 | 0.66 | 0.986343 |
Target: 5'- aGUCGCCUGAcCGuguGAcgCugGGggagAAGUa -3' miRNA: 3'- -CAGUGGACUuGU---CUuaGugCCa---UUCG- -5' |
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18065 | 5' | -47.6 | NC_004680.1 | + | 45464 | 0.66 | 0.984398 |
Target: 5'- uGUUGCCUccACAGggUUAgguaucCGGUAGGUg -3' miRNA: 3'- -CAGUGGAcuUGUCuuAGU------GCCAUUCG- -5' |
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18065 | 5' | -47.6 | NC_004680.1 | + | 33779 | 0.67 | 0.98225 |
Target: 5'- gGUCggGCCgGGACAGcGGUgACGGUucGGGCa -3' miRNA: 3'- -CAG--UGGaCUUGUC-UUAgUGCCA--UUCG- -5' |
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18065 | 5' | -47.6 | NC_004680.1 | + | 48309 | 0.67 | 0.98225 |
Target: 5'- -aCACgUGGGguGAGgcgaUGCGGUGGGCg -3' miRNA: 3'- caGUGgACUUguCUUa---GUGCCAUUCG- -5' |
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18065 | 5' | -47.6 | NC_004680.1 | + | 14490 | 0.67 | 0.979887 |
Target: 5'- cGUCAaauCCUGcacCAGAGUCGuCuGUGAGCg -3' miRNA: 3'- -CAGU---GGACuu-GUCUUAGU-GcCAUUCG- -5' |
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18065 | 5' | -47.6 | NC_004680.1 | + | 3353 | 0.67 | 0.979887 |
Target: 5'- -aCACUUGAACAGGAaacccuuuUCAauguuGUGAGCa -3' miRNA: 3'- caGUGGACUUGUCUU--------AGUgc---CAUUCG- -5' |
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18065 | 5' | -47.6 | NC_004680.1 | + | 19434 | 0.67 | 0.977298 |
Target: 5'- -aCGCCaccACAuGggUgCGCGGUGAGCa -3' miRNA: 3'- caGUGGacuUGU-CuuA-GUGCCAUUCG- -5' |
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18065 | 5' | -47.6 | NC_004680.1 | + | 19053 | 0.67 | 0.974471 |
Target: 5'- -aCGCCuaUGAGCuu-GUCcCGGUGGGCg -3' miRNA: 3'- caGUGG--ACUUGucuUAGuGCCAUUCG- -5' |
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18065 | 5' | -47.6 | NC_004680.1 | + | 17139 | 0.68 | 0.968065 |
Target: 5'- -aCACCUGGGcCAGAGUCGa-GUucGCg -3' miRNA: 3'- caGUGGACUU-GUCUUAGUgcCAuuCG- -5' |
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18065 | 5' | -47.6 | NC_004680.1 | + | 21742 | 0.68 | 0.968065 |
Target: 5'- --gACCaGGACGGGA--ACGGUGAGUg -3' miRNA: 3'- cagUGGaCUUGUCUUagUGCCAUUCG- -5' |
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18065 | 5' | -47.6 | NC_004680.1 | + | 27470 | 0.68 | 0.964467 |
Target: 5'- --uGCCUGGucGCGGGAUCAgUGGaugAAGCa -3' miRNA: 3'- cagUGGACU--UGUCUUAGU-GCCa--UUCG- -5' |
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18065 | 5' | -47.6 | NC_004680.1 | + | 5514 | 0.68 | 0.951994 |
Target: 5'- -gCAuCCgaggGAGCAGAA-C-CGGUAAGCg -3' miRNA: 3'- caGU-GGa---CUUGUCUUaGuGCCAUUCG- -5' |
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18065 | 5' | -47.6 | NC_004680.1 | + | 57234 | 0.69 | 0.947255 |
Target: 5'- --gACCUGGACAGuguugggaCACGGaccUGAGCa -3' miRNA: 3'- cagUGGACUUGUCuua-----GUGCC---AUUCG- -5' |
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18065 | 5' | -47.6 | NC_004680.1 | + | 45241 | 0.69 | 0.931233 |
Target: 5'- uGUCAUCguUGGGCGGAGUCcuauCGGgcgcAAGCg -3' miRNA: 3'- -CAGUGG--ACUUGUCUUAGu---GCCa---UUCG- -5' |
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18065 | 5' | -47.6 | NC_004680.1 | + | 945 | 0.69 | 0.925283 |
Target: 5'- gGUCACCgagGAACAGGuGUCAgagcuugcguuCGGUGuGCu -3' miRNA: 3'- -CAGUGGa--CUUGUCU-UAGU-----------GCCAUuCG- -5' |
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18065 | 5' | -47.6 | NC_004680.1 | + | 34444 | 0.71 | 0.890968 |
Target: 5'- uUCACCUucGCGGAGUguuucgccgcCGCGGUAgucGGCg -3' miRNA: 3'- cAGUGGAcuUGUCUUA----------GUGCCAU---UCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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