Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18235 | 5' | -57.7 | NC_004680.1 | + | 28885 | 0.67 | 0.509533 |
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Target: 5'- cGCg-AUCGCCUCCgguaGCGGuGCcUGCACa -3' miRNA: 3'- uCGagUAGUGGAGG----UGCC-CG-ACGUGg -5' |
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| 18235 | 5' | -57.7 | NC_004680.1 | + | 23700 | 0.67 | 0.530281 |
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Target: 5'- aAGC-CAUaccaaacccCCUCCuaGGGCUGCGCCc -3' miRNA: 3'- -UCGaGUAgu-------GGAGGugCCCGACGUGG- -5' |
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| 18235 | 5' | -57.7 | NC_004680.1 | + | 26178 | 0.66 | 0.56195 |
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Target: 5'- cGUUCca-GCCggcggggCCGCGGGaCUGCACa -3' miRNA: 3'- uCGAGuagUGGa------GGUGCCC-GACGUGg -5' |
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| 18235 | 5' | -57.7 | NC_004680.1 | + | 27998 | 0.66 | 0.572625 |
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Target: 5'- cGUUCAugcggaaaaaguUCGCCUCCuacGCGGGCgauuuggcUGaCACCc -3' miRNA: 3'- uCGAGU------------AGUGGAGG---UGCCCG--------AC-GUGG- -5' |
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| 18235 | 5' | -57.7 | NC_004680.1 | + | 50335 | 0.66 | 0.594109 |
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Target: 5'- gGGCcgaUCAUCACCUCgccgcccugUAUGGGCauugGUAUCg -3' miRNA: 3'- -UCG---AGUAGUGGAG---------GUGCCCGa---CGUGG- -5' |
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| 18235 | 5' | -57.7 | NC_004680.1 | + | 5603 | 0.66 | 0.615718 |
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Target: 5'- cGGCgacgCugCuggUCUGCGGGCggGCACCa -3' miRNA: 3'- -UCGaguaGugG---AGGUGCCCGa-CGUGG- -5' |
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| 18235 | 5' | -57.7 | NC_004680.1 | + | 5995 | 0.66 | 0.615718 |
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Target: 5'- cAGCgCGUCACCgg-GCGGGUguuCACCa -3' miRNA: 3'- -UCGaGUAGUGGaggUGCCCGac-GUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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