Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18252 | 5' | -55.3 | NC_004681.1 | + | 32880 | 0.66 | 0.821861 |
Target: 5'- cGGACUCCUGguaCgaGCgGCccagcGGAUUGCCg -3' miRNA: 3'- -CCUGAGGGUa--GaaCG-CGu----CCUGACGG- -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 31225 | 0.66 | 0.821861 |
Target: 5'- uGAUggCCAUCUUGgGaugcGGugUGCCg -3' miRNA: 3'- cCUGagGGUAGAACgCgu--CCugACGG- -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 39789 | 0.66 | 0.80353 |
Target: 5'- cGGAagggCCaUAUCUuuuUGUGCGGGcaauuGCUGCCu -3' miRNA: 3'- -CCUga--GG-GUAGA---ACGCGUCC-----UGACGG- -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 72576 | 0.66 | 0.794104 |
Target: 5'- cGGAgUCaaCCAU--UGCGC-GGACUGCa -3' miRNA: 3'- -CCUgAG--GGUAgaACGCGuCCUGACGg -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 72252 | 0.66 | 0.784516 |
Target: 5'- uGGACUCCacgaugGCGCGGuauucggcaGCUGCCu -3' miRNA: 3'- -CCUGAGGguagaaCGCGUCc--------UGACGG- -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 17792 | 0.66 | 0.774777 |
Target: 5'- uGGCgaaCCGUCgagGCGUagucGGGAgCUGCCg -3' miRNA: 3'- cCUGag-GGUAGaa-CGCG----UCCU-GACGG- -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 8980 | 0.67 | 0.754887 |
Target: 5'- uGGAC-CUCuucCUUGCGCuGGA-UGCCu -3' miRNA: 3'- -CCUGaGGGua-GAACGCGuCCUgACGG- -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 49615 | 0.67 | 0.724183 |
Target: 5'- aGAUUCCCGUCgucguccgGUGaacgagaAGGAgUGCCg -3' miRNA: 3'- cCUGAGGGUAGaa------CGCg------UCCUgACGG- -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 36892 | 0.67 | 0.724183 |
Target: 5'- aGGACUgCCUAgucaGCGgGGGGCggGCCa -3' miRNA: 3'- -CCUGA-GGGUagaaCGCgUCCUGa-CGG- -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 323 | 0.68 | 0.70326 |
Target: 5'- cGGGCUgCCGgauuUC-UGCG-AGGGCUGCa -3' miRNA: 3'- -CCUGAgGGU----AGaACGCgUCCUGACGg -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 75195 | 0.68 | 0.692693 |
Target: 5'- cGGACUCaCCGUCgacaucCGCcuugAGGACaGCCc -3' miRNA: 3'- -CCUGAG-GGUAGaac---GCG----UCCUGaCGG- -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 48671 | 0.68 | 0.691633 |
Target: 5'- aGGGCguggCCCAcgaUGCaGCAGGgcuggguGCUGCCg -3' miRNA: 3'- -CCUGa---GGGUagaACG-CGUCC-------UGACGG- -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 49585 | 0.68 | 0.682071 |
Target: 5'- aGGAC-CCCGgcgaCUggGCaGUAGGGCuUGCCg -3' miRNA: 3'- -CCUGaGGGUa---GAa-CG-CGUCCUG-ACGG- -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 44296 | 0.69 | 0.628478 |
Target: 5'- uGGACUgCgCAUCggGUGUGGGACUGg- -3' miRNA: 3'- -CCUGAgG-GUAGaaCGCGUCCUGACgg -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 44918 | 0.69 | 0.606996 |
Target: 5'- gGGGCcgCCCGUCUcgaUGuCGUAGGuCUuGCCc -3' miRNA: 3'- -CCUGa-GGGUAGA---AC-GCGUCCuGA-CGG- -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 16412 | 0.7 | 0.585596 |
Target: 5'- cGGCUUCCGUCgauccgGcCGCGGGGaUGCCc -3' miRNA: 3'- cCUGAGGGUAGaa----C-GCGUCCUgACGG- -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 51077 | 0.7 | 0.576011 |
Target: 5'- cGGugUUCCAUUgacgcuuggcgucgGCGUAGGugUaGCCg -3' miRNA: 3'- -CCugAGGGUAGaa------------CGCGUCCugA-CGG- -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 71337 | 0.7 | 0.574948 |
Target: 5'- uGGCUCCg----UGUGCGuGGACUGCCu -3' miRNA: 3'- cCUGAGGguagaACGCGU-CCUGACGG- -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 18927 | 0.7 | 0.553794 |
Target: 5'- aGGCUCCCGUCgcgggaGCGCAGaACgGCUu -3' miRNA: 3'- cCUGAGGGUAGaa----CGCGUCcUGaCGG- -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 22287 | 0.71 | 0.512248 |
Target: 5'- uGGGCgcccugCCCGUCaugGCGCAGGGugaGCCc -3' miRNA: 3'- -CCUGa-----GGGUAGaa-CGCGUCCUga-CGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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