Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18252 | 5' | -55.3 | NC_004681.1 | + | 7552 | 0.71 | 0.481952 |
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Target: 5'- gGGACUgUCGUCUacacgGUGgAGGACgGCCg -3' miRNA: 3'- -CCUGAgGGUAGAa----CGCgUCCUGaCGG- -5' |
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| 18252 | 5' | -55.3 | NC_004681.1 | + | 30063 | 0.71 | 0.481952 |
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Target: 5'- cGGAgCUCCCG-CUgucGCGCAGG-CUcaGCCg -3' miRNA: 3'- -CCU-GAGGGUaGAa--CGCGUCCuGA--CGG- -5' |
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| 18252 | 5' | -55.3 | NC_004681.1 | + | 36107 | 0.75 | 0.329807 |
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Target: 5'- -uGCUCCCAUCgaaUGCGCGGGcaaucuugcGCcGCCa -3' miRNA: 3'- ccUGAGGGUAGa--ACGCGUCC---------UGaCGG- -5' |
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| 18252 | 5' | -55.3 | NC_004681.1 | + | 50378 | 0.75 | 0.314406 |
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Target: 5'- gGGACguggCCCuUCUUGCGguGGGCgcggGaCCa -3' miRNA: 3'- -CCUGa---GGGuAGAACGCguCCUGa---C-GG- -5' |
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| 18252 | 5' | -55.3 | NC_004681.1 | + | 54450 | 0.75 | 0.299556 |
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Target: 5'- aGGCgugCCCA-CUUGCGCAGGuACUuagGCCa -3' miRNA: 3'- cCUGa--GGGUaGAACGCGUCC-UGA---CGG- -5' |
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| 18252 | 5' | -55.3 | NC_004681.1 | + | 3166 | 1.15 | 0.000591 |
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Target: 5'- aGGACUCCCAUCUUGCGCAGGACUGCCa -3' miRNA: 3'- -CCUGAGGGUAGAACGCGUCCUGACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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