Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18252 | 5' | -55.3 | NC_004681.1 | + | 17792 | 0.66 | 0.774777 |
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Target: 5'- uGGCgaaCCGUCgagGCGUagucGGGAgCUGCCg -3' miRNA: 3'- cCUGag-GGUAGaa-CGCG----UCCU-GACGG- -5' |
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| 18252 | 5' | -55.3 | NC_004681.1 | + | 72252 | 0.66 | 0.784516 |
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Target: 5'- uGGACUCCacgaugGCGCGGuauucggcaGCUGCCu -3' miRNA: 3'- -CCUGAGGguagaaCGCGUCc--------UGACGG- -5' |
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| 18252 | 5' | -55.3 | NC_004681.1 | + | 72576 | 0.66 | 0.794104 |
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Target: 5'- cGGAgUCaaCCAU--UGCGC-GGACUGCa -3' miRNA: 3'- -CCUgAG--GGUAgaACGCGuCCUGACGg -5' |
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| 18252 | 5' | -55.3 | NC_004681.1 | + | 39789 | 0.66 | 0.80353 |
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Target: 5'- cGGAagggCCaUAUCUuuuUGUGCGGGcaauuGCUGCCu -3' miRNA: 3'- -CCUga--GG-GUAGA---ACGCGUCC-----UGACGG- -5' |
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| 18252 | 5' | -55.3 | NC_004681.1 | + | 31225 | 0.66 | 0.821861 |
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Target: 5'- uGAUggCCAUCUUGgGaugcGGugUGCCg -3' miRNA: 3'- cCUGagGGUAGAACgCgu--CCugACGG- -5' |
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| 18252 | 5' | -55.3 | NC_004681.1 | + | 32880 | 0.66 | 0.821861 |
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Target: 5'- cGGACUCCUGguaCgaGCgGCccagcGGAUUGCCg -3' miRNA: 3'- -CCUGAGGGUa--GaaCG-CGu----CCUGACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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