Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18252 | 5' | -55.3 | NC_004681.1 | + | 51077 | 0.7 | 0.576011 |
Target: 5'- cGGugUUCCAUUgacgcuuggcgucgGCGUAGGugUaGCCg -3' miRNA: 3'- -CCugAGGGUAGaa------------CGCGUCCugA-CGG- -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 71337 | 0.7 | 0.574948 |
Target: 5'- uGGCUCCg----UGUGCGuGGACUGCCu -3' miRNA: 3'- cCUGAGGguagaACGCGU-CCUGACGG- -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 50378 | 0.75 | 0.314406 |
Target: 5'- gGGACguggCCCuUCUUGCGguGGGCgcggGaCCa -3' miRNA: 3'- -CCUGa---GGGuAGAACGCguCCUGa---C-GG- -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 54450 | 0.75 | 0.299556 |
Target: 5'- aGGCgugCCCA-CUUGCGCAGGuACUuagGCCa -3' miRNA: 3'- cCUGa--GGGUaGAACGCGUCC-UGA---CGG- -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 3166 | 1.15 | 0.000591 |
Target: 5'- aGGACUCCCAUCUUGCGCAGGACUGCCa -3' miRNA: 3'- -CCUGAGGGUAGAACGCGUCCUGACGG- -5' |
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18252 | 5' | -55.3 | NC_004681.1 | + | 32880 | 0.66 | 0.821861 |
Target: 5'- cGGACUCCUGguaCgaGCgGCccagcGGAUUGCCg -3' miRNA: 3'- -CCUGAGGGUa--GaaCG-CGu----CCUGACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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