Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18253 | 5' | -50 | NC_004681.1 | + | 57859 | 0.71 | 0.786202 |
Target: 5'- --gCGGUGUCGCCuGAAGC---UCGGCg -3' miRNA: 3'- uagGUCACAGCGG-UUUCGuuuAGCUG- -5' |
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18253 | 5' | -50 | NC_004681.1 | + | 45109 | 0.71 | 0.775929 |
Target: 5'- -cCCAGUcaUUGCCGAAGCGGG-CGACg -3' miRNA: 3'- uaGGUCAc-AGCGGUUUCGUUUaGCUG- -5' |
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18253 | 5' | -50 | NC_004681.1 | + | 59947 | 0.72 | 0.733378 |
Target: 5'- -cCCGGcaGUCGCCGAGGUGGAUCaaGACa -3' miRNA: 3'- uaGGUCa-CAGCGGUUUCGUUUAG--CUG- -5' |
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18253 | 5' | -50 | NC_004681.1 | + | 66654 | 0.72 | 0.733378 |
Target: 5'- gAUCCGG---CGCCGAAGCGuuuAUCGACc -3' miRNA: 3'- -UAGGUCacaGCGGUUUCGUu--UAGCUG- -5' |
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18253 | 5' | -50 | NC_004681.1 | + | 30986 | 0.73 | 0.655225 |
Target: 5'- cGUCCgcguacagGGUGUCGCgGGgcuccAGCGGGUCGGCg -3' miRNA: 3'- -UAGG--------UCACAGCGgUU-----UCGUUUAGCUG- -5' |
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18253 | 5' | -50 | NC_004681.1 | + | 40564 | 0.76 | 0.509663 |
Target: 5'- -aCCgAGcGUCGCCGAGGCAAAcggCGACa -3' miRNA: 3'- uaGG-UCaCAGCGGUUUCGUUUa--GCUG- -5' |
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18253 | 5' | -50 | NC_004681.1 | + | 4483 | 1.08 | 0.004769 |
Target: 5'- aAUCCAGUGUCGCCAAAGCAAAUCGACu -3' miRNA: 3'- -UAGGUCACAGCGGUUUCGUUUAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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