Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18253 | 5' | -50 | NC_004681.1 | + | 18363 | 0.66 | 0.960757 |
Target: 5'- -cCCGGUGgCGCCAucgacauGGCAGGccaggCGGCg -3' miRNA: 3'- uaGGUCACaGCGGUu------UCGUUUa----GCUG- -5' |
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18253 | 5' | -50 | NC_004681.1 | + | 19115 | 0.66 | 0.960757 |
Target: 5'- uUCCAgGUGUCGCCGucuuGGCGAGc---- -3' miRNA: 3'- uAGGU-CACAGCGGUu---UCGUUUagcug -5' |
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18253 | 5' | -50 | NC_004681.1 | + | 31098 | 0.66 | 0.964548 |
Target: 5'- aGUCCGca--CGCUcaaGGAGCAGAUCGGCa -3' miRNA: 3'- -UAGGUcacaGCGG---UUUCGUUUAGCUG- -5' |
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18253 | 5' | -50 | NC_004681.1 | + | 29849 | 0.66 | 0.964548 |
Target: 5'- --gCAG-GUCGCgGAAGCGc-UCGACa -3' miRNA: 3'- uagGUCaCAGCGgUUUCGUuuAGCUG- -5' |
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18253 | 5' | -50 | NC_004681.1 | + | 15267 | 0.66 | 0.964548 |
Target: 5'- cGUCCA-UGUCGaCAccGAGCAGAcCGGCg -3' miRNA: 3'- -UAGGUcACAGCgGU--UUCGUUUaGCUG- -5' |
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18253 | 5' | -50 | NC_004681.1 | + | 14311 | 0.66 | 0.968075 |
Target: 5'- cUUCAG-GUCGCCGAGGCg---CGGg -3' miRNA: 3'- uAGGUCaCAGCGGUUUCGuuuaGCUg -5' |
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18253 | 5' | -50 | NC_004681.1 | + | 13499 | 0.66 | 0.968075 |
Target: 5'- gGUCCAGgccGUCGCCGcGGUugugcuucUCGAUg -3' miRNA: 3'- -UAGGUCa--CAGCGGUuUCGuuu-----AGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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